論文 - 詳細
| RRC ID | 66712 |
|---|---|
| 著者 | Liu JM, Bao ZH, Cao WW, Han JJ, Zhao J, Kang ZZ, Wang LX, Zhao J. |
| タイトル | Enrichment of Type I Methanotrophs with nirS Genes of Three Emergent Macrophytes in a Eutrophic Wetland in China. |
| ジャーナル | Microbes Environ |
| Abstract |
The pmoA gene, encoding particulate methane monooxygenase in methanotrophs, and nirS and nirK genes, encoding bacterial nitrite reductases, were examined in the root and rhizosphere sediment of three common emergent macrophytes (Phragmites australis, Typha angustifolia, and Scirpus triqueter) and unvegetated sediment from eutrophic Wuliangsuhai Lake in China. Sequencing analyses indicated that 334 out of 351 cloned pmoA sequences were phylogenetically the most closely related to type I methanotrophs (Gammaproteobacteria), and Methylomonas denitrificans-like organisms accounted for 44.4% of the total community. In addition, 244 out of 250 cloned nirS gene sequences belonged to type I methanotrophs, and 31.2% of nirS genes were the most closely related to paddy rice soil clone SP-2-12 in Methylomonas of the total community. Three genera of type I methanotrophs, Methylomonas, Methylobacter, and Methylovulum, were common in both pmoA and nirS clone libraries in each sample. A quantitative PCR (qPCR) analysis demonstrated that the copy numbers of the nirS and nirK genes were significantly higher in rhizosphere sediments than in unvegetated sediments in P. australis and T. angustifolia plants. In the same sample, the nirS gene copy number was significantly higher than that of nirK. Furthermore, type I methanotrophs were localized in the root tissues according to catalyzed reporter deposition-fluorescence in situ hybridization (CARD-FISH). Thus, nirS-carrying type I methanotrophs were enriched in macrophyte root and rhizosphere sediment and are expected to play important roles in carbon/nitrogen cycles in a eutrophic wetland. |
| 巻・号 | 35(1) |
| 公開日 | 2020-1-1 |
| DOI | 10.1264/jsme2.ME19098 |
| PMID | 31969532 |
| PMC | PMC7104278 |
| MeSH | Bacterial Proteins / genetics China Eutrophication* Gammaproteobacteria / classification Gammaproteobacteria / genetics* Gammaproteobacteria / metabolism Gene Dosage Genes, Bacterial / genetics* Lakes / microbiology Magnoliopsida / microbiology* Methane / metabolism Nitrite Reductases / genetics Oxygenases / genetics Plant Roots / microbiology Rhizosphere Soil Microbiology* Wetlands* |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM3867 |