| RRC ID |
6671
|
| Author |
Ohkouchi S, Nishio K, Maeda M, Hitomi K, Adachi H, Maki M.
|
| Title |
Identification and characterization of two penta-EF-hand Ca(2+)-binding proteins in Dictyostelium discoideum.
|
| Journal |
J Biochem
|
| Abstract |
Penta-EF-hand (PEF) proteins such as ALG-2 (apoptosis-linked gene 2 product) and the calpain small subunit are a newly classified family of Ca(2+)-binding proteins that possess five EF-hand-like motifs. We identified two mutually homologous PEF proteins, designated DdPEF-1 and DdPEF-2 (64% amino acid residue identities), in the cellular slime mold Dictyostelium discoideum. Both PEF proteins showed a higher similarity to mammalian ALG-2 and peflin (Group I PEF proteins) than to calpain and sorcin subfamily (Group II PEF proteins) in the first EF-hand (EF-1) regions. Northern blot analyses revealed that DdPEF-1 and DdPEF-2 were constitutively expressed throughout development of Dictyostelium, but their levels of expression were developmentally regulated. In situ hybridization analyses demonstrated that DdPEF-1 was expressed in both the anterior prestalk and the posterior prespore regions of the tipped aggregate, slugs and early culminants. On the other hand, DdPEF-2 was dominantly expressed in the anterior tip region of these multicellular structures. Both PEF proteins were detected as 22-23-kDa proteins in soluble fractions in the presence of EGTA but in particulate fractions in the presence of Ca(2+) by Western blotting using specific monoclonal antibodies. Together with the finding of PEF-like sequences in DNA databases of plants, fungi and protists, our results strongly suggest that Group I PEF proteins are ubiquitously present in all eukaryotes and play important roles in basic cellular functions.
|
| Volume |
130(2)
|
| Pages |
207-15
|
| Published |
2001-8-1
|
| DOI |
10.1093/oxfordjournals.jbchem.a002974
|
| PMID |
11481037
|
| MeSH |
Amino Acid Sequence
Animals
Calcium / metabolism
Calcium-Binding Proteins / chemistry*
Calcium-Binding Proteins / classification
Calcium-Binding Proteins / genetics
Calcium-Binding Proteins / metabolism*
Cell Fractionation
Dictyostelium / chemistry*
Dictyostelium / genetics
EF Hand Motifs*
Gene Expression Regulation, Developmental
In Situ Hybridization
Molecular Sequence Data
Phylogeny
Protozoan Proteins / chemistry
Protozoan Proteins / classification
Protozoan Proteins / genetics
Protozoan Proteins / metabolism
Sequence Alignment
|
| IF |
2.476
|
| Times Cited |
21
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Cellular slime molds |
G00626
G01245 |
