Reference - Detail
| RRC ID | 66758 |
|---|---|
| Author | Shiozawa A, Kajiwara C, Ishii Y, Tateda K. |
| Title | N-acetyl-cysteine mediates protection against Mycobacterium avium through induction of human β-defensin-2 in a mouse lung infection model. |
| Journal | Microbes Infect |
| Abstract |
Mycobacterium avium complex is a causative organism for refractory diseases. In this study, we examined the effects of N-acetyl-cysteine on M. avium infection in vitro and in vivo. N-acetyl-cysteine treatment suppressed the growth of M. avium in A549 cells in a concentration-dependent manner. This effect was related to the induction of the antibacterial peptide human β-defensin-2. In a mouse model, N-acetyl-cysteine treatment significantly reduced the number of bacteria in the lungs and induced murine β-defensin-3. In interleukin-17-deficient mice, the effects of N-acetyl-cysteine disappeared, indicating that these mechanisms may be mediated by interleukin-17. Moreover, an additional reduction in bacterial load was observed in mice administered N-acetyl-cysteine in combination with clarithromycin. Our findings demonstrate the potent antimycobacterial effects of N-acetyl-cysteine against M. avium by inducing antimicrobial peptide, suggesting that N-acetyl-cysteine may have applications as an alternative to classical treatment regimens. |
| Volume | 22(10) |
| Pages | 567-575 |
| Published | 2020-1-1 |
| DOI | 10.1016/j.micinf.2020.08.003 |
| PII | S1286-4579(20)30150-7 |
| PMID | 32882411 |
| MeSH | A549 Cells Acetylcysteine / pharmacology* Acetylcysteine / therapeutic use Animals Antitubercular Agents / pharmacology* Antitubercular Agents / therapeutic use Bacterial Load / drug effects Disease Models, Animal Humans Interleukin-17 / metabolism Lung / drug effects Lung / microbiology Mice Mycobacterium avium / drug effects* Mycobacterium avium / growth & development Mycobacterium avium-intracellulare Infection / drug therapy* Mycobacterium avium-intracellulare Infection / metabolism Mycobacterium avium-intracellulare Infection / microbiology RAW 264.7 Cells Signal Transduction beta-Defensins / metabolism* |
| Resource | |
| General Microbes | JCM15430 |