論文 - 詳細
| RRC ID | 66771 |
|---|---|
| 著者 | Sasaki K, Sasaki D, Hannya A, Tsubota J, Kondo A. |
| タイトル | In vitro human colonic microbiota utilises D-β-hydroxybutyrate to increase butyrogenesis. |
| ジャーナル | Sci Rep |
| Abstract |
The ketone body D-β-hydroxybutyrate (DBHB) has gained attention owing to its cellular signalling function; however, its effect on the human colonic microbiota remains unclear. Here, DBHB dynamics in the human colon were investigated using an in vitro colonic microbiota model, which maintained most of the operational taxonomic units detected in the original faeces. Over 54% of 0.41% (w/v) DBHB was metabolised by microbiota models originating from seven faecal samples after 30 h of fermentation (regarded as DBHB utilisers); however, <19% of DBHB was metabolised by microbiota models from five faecal samples (regarded as non-utilisers of DBHB). In utilisers, DBHB administration increased the relative abundance of the genus Coprococcus, correlated with increased butyrogenesis. Increased butyrogenesis was not observed in DBHB non-utilisers. Based on PICRUSt analysis, the relative abundance of β-hydroxybutyrate dehydrogenase was maintained in microbiota models from DBHB utilisers following DBHB administration; however, it decreased in microbiota models from non-utilisers. After 21 h of fermentation, the intracellular glutamate concentration, which is indicative of growth, showed a positive correlation with DBHB utilisation (R2 = 0.70). Human colonic microbiotas with high growth activity demonstrate efficient utilisation of DBHB for increased butyrate production, which affords health benefits. |
| 巻・号 | 10(1) |
| ページ | 8516 |
| 公開日 | 2020-5-22 |
| DOI | 10.1038/s41598-020-65561-5 |
| PII | 10.1038/s41598-020-65561-5 |
| PMID | 32444846 |
| PMC | PMC7244492 |
| MeSH | 3-Hydroxybutyric Acid / metabolism* Butyrates / metabolism* Clostridiales / genetics Clostridiales / isolation & purification Clostridiales / metabolism* Colon / metabolism* Colon / microbiology DNA, Bacterial / genetics Feces / microbiology* Gastrointestinal Microbiome* Humans In Vitro Techniques Metabolome |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 11 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM31264 JCM31265 |