Reference - Detail
|Author||Imai K, Kijima T, Noda Y, Sutoh K, Yoda K, Adachi H.|
|Title||A Rho GDP-dissociation inhibitor is involved in cytokinesis of Dictyostelium.|
|Journal||Biochem. Biophys. Res. Commun.|
Homology searches toward the EST databases of Dictyostelium discoideum identified two putative Rho GDP-dissociation inhibitors (RhoGDIs), RhoGDI1 and RhoGDI2. In this study, the roles of RhoGDI1 in cytokinesis were examined. The RhoGDI1-null Dictyostelium strains produced by homologous recombination were viable but generated multinucleate giant cells in suspension culture, suggesting that RhoGDI1 is involved in cytokinesis. The expression of green fluorescent protein (GFP)-tagged RhoGDI1 complemented the defects of the RhoGDI1-null cells, and the GFP-RhoGDI1 is predominantly present in cytoplasm of the cell-like yeast RhoGDI. Of 15 Rho family GTPases in Dictyostelium currently known, Dictyostelium versions of Rac1 proteins (Rac1A, Rac1B, and Rac1C) and RacE that are reportedly involved in Dictyostelium cytokinesis, showed two-hybrid interactions with RhoGDI1 as well as human and yeast Cdc42. These results suggest that RhoGDI1 is involved in cytokinesis of Dicytostelium through the regulation of Rho family GTPases Rac1s and/or RacE.
|MeSH||Amino Acid Motifs Amino Acid Sequence Animals Cell Division / physiology* Dictyostelium / cytology Dictyostelium / genetics Dictyostelium / physiology* Expressed Sequence Tags Genes, Reporter Genetic Complementation Test Guanine Nucleotide Dissociation Inhibitors / chemistry Guanine Nucleotide Dissociation Inhibitors / genetics Guanine Nucleotide Dissociation Inhibitors / metabolism* Humans Molecular Sequence Data Protozoan Proteins / chemistry Protozoan Proteins / genetics Protozoan Proteins / metabolism Recombinant Fusion Proteins / metabolism Sequence Alignment Two-Hybrid System Techniques rac GTP-Binding Proteins / metabolism rho GTP-Binding Proteins / genetics rho GTP-Binding Proteins / metabolism*|
|WOS Category||BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY|
|Cellular slime molds||G02219|