Reference - Detail
| RRC ID | 66800 |
|---|---|
| Author | Dou TY, Chen J, Liu C. |
| Title | Isolation and subunit compositions of the xylanosome complexes produced by Cellulosimicrobium species. |
| Journal | Enzyme Microb Technol |
| Abstract |
Cellulosimicrobium cellulans, which is type species of the genus Cellulosimicrobium, produces xylanase predominant nanoscale multienzyme complexes, i.e., xylanosomes, when grown on water-insoluble polysaccharides. Here, we report on the isolation of similar multienzyme complexes (MECs) produced by two other species in genus Cellulosimicrobium (Cellulosimicrobium funkei and Cellulosimicrobium terreum). Functional studies and subunit structure identifications using genomic sequencing and proteomic techniques were also performed. When compared with the xylanosomes produced by C. cellulans F16, the isolated MECs showed a larger particle size and shared at least three conserved multidomain proteins. In addition, they also exhibited different enzymatic activities and subunit compositions, which indicates diverse capability and strategies in degrading hemicelluloses. |
| Volume | 133 |
| Pages | 109445 |
| Published | 2020-2-1 |
| DOI | 10.1016/j.enzmictec.2019.109445 |
| PII | S0141-0229(19)30183-8 |
| PMID | 31874683 |
| MeSH | Actinobacteria / enzymology* Actinobacteria / genetics* Carbohydrate Metabolism Multienzyme Complexes / chemistry* Multienzyme Complexes / genetics* Multienzyme Complexes / isolation & purification Phylogeny Proteomics RNA, Ribosomal, 16S / genetics Xylans / metabolism* |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM14302 JCM15619 |