論文 - 詳細
| RRC ID | 66845 |
|---|---|
| 著者 | Yamaoka Y, Miyakawa K, Jeremiah SS, Funabashi R, Okudela K, Kikuchi S, Katada J, Wada A, Takei T, Nishi M, Shimizu K, Ozawa H, Usuku S, Kawakami C, Tanaka N, Morita T, Hayashi H, Mitsui H, Suzuki K, Aizawa D, Yoshimura Y, Miyazaki T, Yamazaki E, Suzuki T, Kimura H, Shimizu H, Okabe N, Hasegawa H, Ryo A. |
| タイトル | Highly specific monoclonal antibodies and epitope identification against SARS-CoV-2 nucleocapsid protein for antigen detection tests. |
| ジャーナル | Cell Rep Med |
| Abstract |
The ongoing coronavirus disease 2019 (COVID-19) pandemic is a major global public health concern. Although rapid point-of-care testing for detecting viral antigen is important for management of the outbreak, the current antigen tests are less sensitive than nucleic acid testing. In our current study, we produce monoclonal antibodies (mAbs) that exclusively react with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and exhibit no cross-reactivity with other human coronaviruses, including SARS-CoV. Molecular modeling suggests that the mAbs bind to epitopes present on the exterior surface of the nucleocapsid, making them suitable for detecting SARS-CoV-2 in clinical samples. We further select the optimal pair of anti-SARS-CoV-2 nucleocapsid protein (NP) mAbs using ELISA and then use this mAb pair to develop immunochromatographic assay augmented with silver amplification technology. Our mAbs recognize the variants of concern (501Y.V1-V3) that are currently in circulation. Because of their high performance, the mAbs of this study can serve as good candidates for developing antigen detection kits for COVID-19. |
| 巻・号 | 2(6) |
| ページ | 100311 |
| 公開日 | 2021-6-15 |
| DOI | 10.1016/j.xcrm.2021.100311 |
| PII | S2666-3791(21)00154-3 |
| PMID | 34027498 |
| PMC | PMC8126173 |
| MeSH | Animals Antibodies, Monoclonal / immunology* Antigen-Antibody Reactions COVID-19 / pathology COVID-19 / virology Coronavirus Nucleocapsid Proteins / genetics Coronavirus Nucleocapsid Proteins / immunology* Coronavirus Nucleocapsid Proteins / metabolism Epitopes / immunology* Humans Immunization Immunoassay / methods* Mice Mice, Inbred BALB C Phosphoproteins / genetics Phosphoproteins / immunology Phosphoproteins / metabolism Point-of-Care Systems SARS-CoV-2 / isolation & purification SARS-CoV-2 / metabolism* Silver / chemistry |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 54 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MRC-5(RCB0211) |