RRC ID 66845
Author Yamaoka Y, Miyakawa K, Jeremiah SS, Funabashi R, Okudela K, Kikuchi S, Katada J, Wada A, Takei T, Nishi M, Shimizu K, Ozawa H, Usuku S, Kawakami C, Tanaka N, Morita T, Hayashi H, Mitsui H, Suzuki K, Aizawa D, Yoshimura Y, Miyazaki T, Yamazaki E, Suzuki T, Kimura H, Shimizu H, Okabe N, Hasegawa H, Ryo A.
Title Highly specific monoclonal antibodies and epitope identification against SARS-CoV-2 nucleocapsid protein for antigen detection tests.
Journal Cell Rep Med
Abstract The ongoing coronavirus disease 2019 (COVID-19) pandemic is a major global public health concern. Although rapid point-of-care testing for detecting viral antigen is important for management of the outbreak, the current antigen tests are less sensitive than nucleic acid testing. In our current study, we produce monoclonal antibodies (mAbs) that exclusively react with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and exhibit no cross-reactivity with other human coronaviruses, including SARS-CoV. Molecular modeling suggests that the mAbs bind to epitopes present on the exterior surface of the nucleocapsid, making them suitable for detecting SARS-CoV-2 in clinical samples. We further select the optimal pair of anti-SARS-CoV-2 nucleocapsid protein (NP) mAbs using ELISA and then use this mAb pair to develop immunochromatographic assay augmented with silver amplification technology. Our mAbs recognize the variants of concern (501Y.V1-V3) that are currently in circulation. Because of their high performance, the mAbs of this study can serve as good candidates for developing antigen detection kits for COVID-19.
Volume 2(6)
Pages 100311
Published 2021-6-15
DOI 10.1016/j.xcrm.2021.100311
PII S2666-3791(21)00154-3
PMID 34027498
PMC PMC8126173
MeSH Animals Antibodies, Monoclonal / immunology* Antigen-Antibody Reactions COVID-19 / pathology COVID-19 / virology Coronavirus Nucleocapsid Proteins / genetics Coronavirus Nucleocapsid Proteins / immunology* Coronavirus Nucleocapsid Proteins / metabolism Epitopes / immunology* Humans Immunization Immunoassay / methods* Mice Mice, Inbred BALB C Phosphoproteins / genetics Phosphoproteins / immunology Phosphoproteins / metabolism Point-of-Care Systems SARS-CoV-2 / isolation & purification SARS-CoV-2 / metabolism* Silver / chemistry
Human and Animal Cells MRC-5(RCB0211)