| RRC ID |
66866
|
| 著者 |
Ishida-Ishihara S, Akiyama M, Furusawa K, Naguro I, Ryuno H, Sushida T, Ishihara S, Haga H.
|
| タイトル |
Osmotic gradients induce stable dome morphogenesis on extracellular matrix.
|
| ジャーナル |
J Cell Sci
|
| Abstract |
One of the fundamental processes in morphogenesis is dome formation, but many of the mechanisms involved are unexplored. Previous in vitro studies showed that an osmotic gradient is the driving factor of dome formation. However, these investigations were performed without extracellular matrix (ECM), which provides structural support to morphogenesis. With the use of ECM, we observed that basal hypertonic stress induced stable domes in vitro that have not been seen in previous studies. These domes developed as a result of ECM swelling via aquaporin water transport activity. Based on computer simulation, uneven swelling, with a positive feedback between cell stretching and enhanced water transport, was a cause of dome formation. These results indicate that osmotic gradients induce dome morphogenesis via both enhanced water transport activity and subsequent ECM swelling.
|
| 巻・号 |
133(14)
|
| 公開日 |
2020-7-27
|
| DOI |
10.1242/jcs.243865
|
| PII |
jcs.243865
|
| PMID |
32576662
|
| MeSH |
Computer Simulation
Extracellular Matrix*
Morphogenesis
Osmosis
Osmotic Pressure
|
| IF |
4.573
|
| リソース情報 |
| ヒト・動物細胞 |
MDCK(RCB0995) |
