RRC ID 66886
Author Kariya K, Murata K, Kokubo Y, Ube N, Ueno K, Yabuta Y, Teraishi M, Okumoto Y, Mori N, Ishihara A.
Title Variation of diterpenoid phytoalexin oryzalexin A production in cultivated and wild rice.
Journal Phytochemistry
Abstract Rice (Oryza sativa) leaves accumulate phytoalexins in response to pathogen attack. The major phytoalexins in rice are diterpenoids such as oryzalexins, momilactones, and phytocassanes. We measured the amount of oryzalexin A in leaves irradiated by UV light, treated with jasmonic acid, or inoculated with conidia of Bipolaris oryzae in the japonica cultivar Nipponbare and the indica cultivar Kasalath. Nipponbare leaves accumulated oryzalexin A at a high concentration, but Kasalath leaves did not. The locus responsible for this difference was mapped using backcrossed inbred lines and chromosome substitution lines. A region on Chr. 12 containing the KSL10 gene was responsible for the deficiency in oryzalexin A in the Kasalath cultivar. The amount of KSL10 transcript increased in Nipponbare leaves but not in Kasalath leaves in response to UV light irradiation, indicating that the suppressed expression of KSL10 caused the deficiency of oryzalexin A in Kasalath. We analyzed oryzalexin A accumulation in UV light-irradiated leaves of cultivars in the world rice core collection. There were cultivars that accumulated oryzalexin A and those that did not, and both of these chemotypes were found in japonica and indica subspecies. Furthermore, these chemotypes were found in the wild rice species Oryza rufipogon. The phylogenetic relationship of KSL10 sequences was not correlated to oryzalexin A chemotypes. These findings suggested that the biosynthesis of oryzalexin A was acquired by a common ancestor of O. rufipogon and was lost multiple times during the evolutionary process.
Volume 166
Pages 112057
Published 2019-10-1
DOI 10.1016/j.phytochem.2019.112057
PII S0031-9422(19)30336-X
PMID 31306913
IF 2.905
Resource
Rice W0120, W0130, W0138, W0164, W0593, W0596, W0610, W0625, W1230, W1292, W1294, W1295, W2113, W2115, W2117