RRC ID 66890
Author Murata K, Kitano T, Yoshimoto R, Takata R, Ube N, Ueno K, Ueno M, Yabuta Y, Teraishi M, Holland CK, Jander G, Okumoto Y, Mori N, Ishihara A.
Title Natural variation in the expression and catalytic activity of a naringenin 7-O-methyltransferase influences antifungal defenses in diverse rice cultivars.
Journal Plant J
Abstract Phytoalexins play a pivotal role in plant-pathogen interactions. Whereas leaves of rice (Oryza sativa) cultivar Nipponbare predominantly accumulated the phytoalexin sakuranetin after jasmonic acid induction, only very low amounts accumulated in the Kasalath cultivar. Sakuranetin is synthesized from naringenin by naringenin 7-O-methyltransferase (NOMT). Analysis of chromosome segment substitution lines and backcrossed inbred lines suggested that NOMT is the underlying cause of differential phytoalexin accumulation between Nipponbare and Kasalath. Indeed, both NOMT expression and NOMT enzymatic activity are lower in Kasalath than in Nipponbare. We identified a proline to threonine substitution in Kasalath relative to Nipponbare NOMT as the main cause of the lower enzymatic activity. Expanding this analysis to rice cultivars with varying amounts of sakuranetin collected from around the world showed that NOMT induction is correlated with sakuranetin accumulation. In bioassays with Pyricularia oryzae, Gibberella fujikuroi, Bipolaris oryzae, Burkholderia glumae, Xanthomonas oryzae, Erwinia chrysanthemi, Pseudomonas syringae, and Acidovorax avenae, naringenin was more effective against bacterial pathogens and sakuranetin was more effective against fungal pathogens. Therefore, the relative amounts of naringenin and sakuranetin may provide protection against specific pathogen profiles in different rice-growing environments. In a dendrogram of NOMT genes, those from low-sakuranetin-accumulating cultivars formed at least two clusters, only one of which involves the proline to threonine mutation, suggesting that the low sakuranetin chemotype was acquired more than once in cultivated rice. Strains of the wild rice species Oryza rufipogon also exhibited differential sakuranetin accumulation, indicating that this metabolic diversity predates rice domestication.
Volume 101(5)
Pages 1103-1117
Published 2020-3-1
DOI 10.1111/tpj.14577
PMID 31630460
MeSH Antifungal Agents / pharmacology* Ascomycota / drug effects Burkholderia / drug effects Comamonadaceae / drug effects Cyclopentanes / metabolism* Flavanones / metabolism Flavonoids / metabolism* Fusarium / drug effects Genetic Variation Methyltransferases / genetics* Methyltransferases / metabolism Oryza / enzymology* Oryza / genetics Oryza / immunology Oryza / microbiology Oxylipins / metabolism* Plant Diseases / immunology* Plant Diseases / microbiology Xanthomonas / drug effects
IF 6.141
Rice W0120, W0130, W0138, W0596, W0610, W1230, W1292, W1294, W1295, W2113, W2115, W2117