論文 - 詳細
| RRC ID | 66997 |
|---|---|
| 著者 | Uesugi S, Hakozaki M, Kanno Y, Takahashi H, Kudo Y, Kimura KI, Yamada H, Yano A. |
| タイトル | A yeast-based screening system identified bakkenolide B contained in Petasites japonicus as an inhibitor of interleukin-2 production in a human T cell line. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
Ca2+ signaling is related to various diseases such as allergies, diabetes, and cancer. We explored Ca2+ signaling inhibitors in natural resources using a yeast-based screening method, and found bakkenolide B from the flower buds of edible wild plant, Petasites japonicus, using the YNS17 strain (zds1Δ erg3Δ pdr1/3Δ). Bakkenolide B exhibited growth-restoring activity against the YNS17 strain and induced Li+ sensitivity of wild-type yeast cells, suggesting that it inhibits the calcineurin pathway. Additionally, bakkenolide B inhibited interleukin-2 production at gene and protein levels in Jurkat cells, a human T cell line, but not the in vitro phosphatase activity of human recombinant calcineurin, an upstream regulator of interleukin-2 production. Furthermore, bakkenolide A showed weak activity in YNS17 and Jurkat cells compared with bakkenolide B. These findings revealed new biological effects and the structure-activity relationships of bakkenolides contained in Petasites japonicus as inhibitors of interleukin-2 production in human T cells. |
| 巻・号 | 85(10) |
| ページ | 2153-2160 |
| 公開日 | 2021-9-22 |
| DOI | 10.1093/bbb/zbab130 |
| PII | 6319492 |
| PMID | 34251393 |
| MeSH | Saccharomyces cerevisiae* Sesquiterpenes* |
| IF | 1.516 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Jurkat(RCB3052) |