RRC ID |
66997
|
著者 |
Uesugi S, Hakozaki M, Kanno Y, Takahashi H, Kudo Y, Kimura KI, Yamada H, Yano A.
|
タイトル |
A yeast-based screening system identified bakkenolide B contained in Petasites japonicus as an inhibitor of interleukin-2 production in a human T cell line.
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ジャーナル |
Biosci Biotechnol Biochem
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Abstract |
Ca2+ signaling is related to various diseases such as allergies, diabetes, and cancer. We explored Ca2+ signaling inhibitors in natural resources using a yeast-based screening method, and found bakkenolide B from the flower buds of edible wild plant, Petasites japonicus, using the YNS17 strain (zds1Δ erg3Δ pdr1/3Δ). Bakkenolide B exhibited growth-restoring activity against the YNS17 strain and induced Li+ sensitivity of wild-type yeast cells, suggesting that it inhibits the calcineurin pathway. Additionally, bakkenolide B inhibited interleukin-2 production at gene and protein levels in Jurkat cells, a human T cell line, but not the in vitro phosphatase activity of human recombinant calcineurin, an upstream regulator of interleukin-2 production. Furthermore, bakkenolide A showed weak activity in YNS17 and Jurkat cells compared with bakkenolide B. These findings revealed new biological effects and the structure-activity relationships of bakkenolides contained in Petasites japonicus as inhibitors of interleukin-2 production in human T cells.
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巻・号 |
85(10)
|
ページ |
2153-2160
|
公開日 |
2021-9-22
|
DOI |
10.1093/bbb/zbab130
|
PII |
6319492
|
PMID |
34251393
|
MeSH |
Saccharomyces cerevisiae*
Sesquiterpenes*
|
IF |
1.516
|
リソース情報 |
ヒト・動物細胞 |
Jurkat(RCB3052) |