RRC ID 67074
Author Ma L, Sakamoto Y, Kanai A, Otsuka H, Takahashi A, Kakimi K, Imai T, Shimokawa T.
Title Characterization of a Novel Murine Colon Carcinoma Subline with High-Metastatic Activity Established by In Vivo Selection Method.
Journal Int J Mol Sci
Abstract The establishment of cancer cell lines, which have different metastatic abilities compared with the parental cell, is considered as an effective approach to investigate mechanisms of metastasis. A highly metastatic potential mouse colon cancer cell subline, Colon-26MGS, was derived from the parental cell line Colon-26 by in vivo selection using continuous subcutaneous implanting to immunocompetent mice. To clarify the mechanisms involved in the enhancement of metastasis, morphological characteristics, cell proliferation, and gene expression profiles were compared between Colon-26MGS and the parental cell. Colon-26MGS showed over 10 times higher metastatic ability compared with the parental cell, but there were no differences in morphological characteristics and in vitro proliferation rates. In addition, the Colon-26MGS-bearing mice exhibited no marked change of splenocyte population and lung pre-metastatic niche with tumor-free mice, but there were significant differences compared to Colon-26-bearing mice. RNA-seq analyses indicated that immune costimulatory molecules were significantly up-regulated in Colon-26MGS. These results suggest that Colon-26MGS showed not only higher metastatic activity, but also less induction property of host immune response compared to parental Colon-26. Colon-26MGS has proven to be a novel useful tool for studying multiple mechanisms involving metastasis enhancement.
Volume 21(8)
Published 2020-4-18
DOI 10.3390/ijms21082829
PII ijms21082829
PMID 32325684
PMC PMC7215277
MeSH Animals Carcinoma / genetics Carcinoma / metabolism* Carcinoma / pathology Carcinoma / secondary* Cell Line, Tumor* Cell Movement / genetics* Cell Proliferation / genetics* Colonic Neoplasms / genetics Colonic Neoplasms / metabolism* Colonic Neoplasms / pathology Female Gene Expression Regulation, Neoplastic / genetics Immunohistochemistry Lung Neoplasms / genetics Lung Neoplasms / metabolism* Lung Neoplasms / pathology Mice Mice, Inbred BALB C RNA-Seq
IF 4.556
Human and Animal Cells LM8(RCB1450)