論文 - 詳細
| RRC ID | 67086 |
|---|---|
| 著者 | Ueno M, Maeshige N, Hirayama Y, Yamaguchi A, Ma X, Uemura M, Kondo H, Fujino H. |
| タイトル | Pulsed ultrasound prevents lipopolysaccharide-induced muscle atrophy through inhibiting p38 MAPK phosphorylation in C2C12 myotubes. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
OBJECTIVE:Inflammation contributes to skeletal muscle atrophy via protein degradation induced by p38 mitogen-activated protein kinase (MAPK) phosphorylation. Meanwhile, pulsed ultrasound irradiation provides the mechanical stimulation to the target tissue, and has been reported to show anti-inflammatory effects. This study investigated the preventive effects of pulsed ultrasound irradiation on muscle atrophy induced by lipopolysaccharide (LPS) in C2C12 myotubes. METHODS:C2C12 myotubes were used in this research. The pulsed ultrasound (a frequency of 3 MHz, duty cycle of 20%, intensity of 0.5 W/cm2) was irradiated to myotube before LPS administration. RESULTS:The LPS increased phosphorylation of p38 MAPK and decreased the myofibril and myosin heavy chain protein (P < 0.05), followed by atrophy in C2C12 myotubes. The pulsed ultrasound irradiation attenuated p38 MAPK phosphorylation and myotube atrophy induced by LPS (P < 0.05). CONCLUSIONS:Pulsed ultrasound irradiation has the preventive effects on inflammation-induced muscle atrophy through inhibiting phosphorylation of p38 MAPK. |
| 巻・号 | 570 |
| ページ | 184-190 |
| 公開日 | 2021-9-17 |
| DOI | 10.1016/j.bbrc.2021.07.039 |
| PII | S0006-291X(21)01076-7 |
| PMID | 34293592 |
| MeSH | Animals Cell Line Interleukin-1 Receptor-Associated Kinases / metabolism Lipopolysaccharides Mice Muscle Fibers, Skeletal / enzymology* Muscle Fibers, Skeletal / pathology* Muscle Proteins / metabolism Muscular Atrophy / chemically induced Muscular Atrophy / enzymology* Muscular Atrophy / pathology* Muscular Atrophy / prevention & control Phosphorylation SKP Cullin F-Box Protein Ligases / metabolism Ultrasonic Waves* p38 Mitogen-Activated Protein Kinases / metabolism* |
| IF | 2.985 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |