Reference - Detail
| RRC ID | 67112 |
|---|---|
| Author | Ouwendijk WJD, Depledge DP, Rajbhandari L, Lenac Rovis T, Jonjic S, Breuer J, Venkatesan A, Verjans GMGM, Sadaoka T. |
| Title | Varicella-zoster virus VLT-ORF63 fusion transcript induces broad viral gene expression during reactivation from neuronal latency. |
| Journal | Nat Commun |
| Abstract |
Varicella-zoster virus (VZV) establishes lifelong neuronal latency in most humans world-wide, reactivating in one-third to cause herpes zoster and occasionally chronic pain. How VZV establishes, maintains and reactivates from latency is largely unknown. VZV transcription during latency is restricted to the latency-associated transcript (VLT) and RNA 63 (encoding ORF63) in naturally VZV-infected human trigeminal ganglia (TG). While significantly more abundant, VLT levels positively correlated with RNA 63 suggesting co-regulated transcription during latency. Here, we identify VLT-ORF63 fusion transcripts and confirm VLT-ORF63, but not RNA 63, expression in human TG neurons. During in vitro latency, VLT is transcribed, whereas VLT-ORF63 expression is induced by reactivation stimuli. One isoform of VLT-ORF63, encoding a fusion protein combining VLT and ORF63 proteins, induces broad viral gene transcription. Collectively, our findings show that VZV expresses a unique set of VLT-ORF63 transcripts, potentially involved in the transition from latency to lytic VZV infection. |
| Volume | 11(1) |
| Pages | 6324 |
| Published | 2020-12-10 |
| DOI | 10.1038/s41467-020-20031-4 |
| PII | 10.1038/s41467-020-20031-4 |
| PMID | 33303747 |
| PMC | PMC7730162 |
| MeSH | Anisomycin / pharmacology Gene Expression Regulation, Viral* Herpesvirus 3, Human / genetics* Humans JNK Mitogen-Activated Protein Kinases / metabolism Open Reading Frames / genetics Protein Isoforms / genetics Protein Isoforms / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism Sensory Receptor Cells / virology* Transcription, Genetic / drug effects Trigeminal Ganglion / pathology Trigeminal Ganglion / virology Viral Proteins / genetics* Viral Proteins / metabolism Virus Activation / genetics* Virus Latency / genetics* |
| IF | 12.121 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 15 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | CS-CA-MCS (RDB05963) pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394) |