論文 - 詳細
| RRC ID | 67118 |
|---|---|
| 著者 | Nakayasu M, Umemoto N, Akiyama R, Ohyama K, Lee HJ, Miyachi H, Watanabe B, Muranaka T, Saito K, Sugimoto Y, Mizutani M. |
| タイトル | Characterization of C-26 aminotransferase, indispensable for steroidal glycoalkaloid biosynthesis. |
| ジャーナル | Plant J |
| Abstract |
Steroidal glycoalkaloids (SGAs) are toxic specialized metabolites found in members of the Solanaceae, such as potato (Solanum tuberosum) and tomato (S. lycopersicum). Major potato SGAs are α-solanine and α-chaconine, which are biosynthesized from cholesterol. Previously, we characterized two cytochrome P450 monooxygenases and a 2-oxoglutarate-dependent dioxygenase that function in hydroxylation at the C-22, C-26, and C-16α positions, while an aminotransferase responsible for the introduction of a nitrogen moiety into the steroidal skeleton remains uncharacterized. Here, we show that PGA4 encoding a putative γ-aminobutyrate aminotransferase is involved in SGA biosynthesis in potatoes. The PGA4 transcript was expressed at high levels in tuber sprouts, in which SGAs are abundant. Silencing the PGA4 gene decreased potato SGA levels and instead caused the accumulation of furostanol saponins. Analysis of the tomato PGA4 ortholog, GAME12, essentially provided the same results. Recombinant PGA4 protein exhibited catalysis of transamination at the C-26 position of 22-hydroxy-26-oxocholesterol using γ-aminobutyric acid as an amino donor. S. stipuloideum (PI 498120), which is a tuber-bearing wild potato species lacking SGA, was found to have a defective PGA4 gene expressing the truncated transcripts, and transformation of PI 498120 with functional PGA4 resulted in complementation of SGA production. These findings indicate that PGA4 is a key enzyme for transamination in SGA biosynthesis. The disruption of PGA4 function by genome editing will be a viable approach for accumulating valuable steroidal saponins in SGA-free potatoes. |
| 巻・号 | 108(1) |
| ページ | 81-92 |
| 公開日 | 2021-10-1 |
| DOI | 10.1111/tpj.15426 |
| PMID | 34273198 |
| MeSH | 4-Aminobutyrate Transaminase / genetics 4-Aminobutyrate Transaminase / metabolism* Gene Editing Hydroxylation Ketocholesterols / biosynthesis Ketocholesterols / chemistry Plant Proteins / genetics Plant Proteins / metabolism Plant Tubers / enzymology Plant Tubers / genetics Plant Tubers / physiology Saponins / biosynthesis Saponins / chemistry Solanine / analogs & derivatives* Solanine / chemistry Solanine / metabolism Solanum tuberosum / enzymology Solanum tuberosum / genetics* Solanum tuberosum / physiology |
| IF | 6.141 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| トマト | TOMJPF00001 (Micro-Tom) |