| RRC ID |
67122
|
| 著者 |
Miyazaki S, Tashiro F, Tsuchiya T, Sasaki K, Miyazaki JI.
|
| タイトル |
Establishment of a long-term stable β-cell line and its application to analyze the effect of Gcg expression on insulin secretion.
|
| ジャーナル |
Sci Rep
|
| Abstract |
A pancreatic β-cell line MIN6 was previously established in our lab from an insulinoma developed in an IT6 transgenic mouse expressing the SV40 T antigen in β-cells. This cell line has been widely used for in vitro analysis of β-cell function, but tends to lose the mature β-cell features, including glucose-stimulated insulin secretion (GSIS), in long-term culture. The aim of this study was to develop a stable β-cell line that retains the characteristics of mature β-cells. Considering that mice derived from a cross between C3H and C57BL/6 strains are known to exhibit higher insulin secretory capacity than C57BL/6 mice, an IT6 male mouse of this hybrid background was used to isolate insulinomas, which were independently cultured. After 7 months of continuous culturing, we obtained the MIN6-CB4 β-cell line, which stably maintains its GSIS. It has been noted that β-cell lines express the glucagon (Gcg) gene at certain levels. MIN6-CB4 cells were utilized to assess the effects of differential Gcg expression on β-cell function. Our data show the functional importance of Gcg expression and resulting basal activation of the GLP-1 receptor in β-cells. MIN6-CB4 cells can serve as an invaluable tool for studying the regulatory mechanisms of insulin secretion, such as the GLP-1/cAMP signaling, in β-cells.
|
| 巻・号 |
11(1)
|
| ページ |
477
|
| 公開日 |
2021-1-12
|
| DOI |
10.1038/s41598-020-79992-7
|
| PII |
10.1038/s41598-020-79992-7
|
| PMID |
33436850
|
| PMC |
PMC7804151
|
| MeSH |
Animals
Female
Glucagon / physiology*
Glucagon-Like Peptide-1 Receptor / metabolism*
Insulin Secretion*
Insulin-Secreting Cells / cytology
Insulin-Secreting Cells / metabolism*
Insulinoma / metabolism*
Insulinoma / pathology
Male
Mice
Mice, Inbred C3H
Mice, Inbred C57BL
Mice, Knockout
Pancreatic Neoplasms / metabolism*
Pancreatic Neoplasms / pathology
|
| IF |
3.998
|
| リソース情報 |
| 遺伝子材料 |
CS-CDF-CG-PRE (RDB04379)
pCMV-VSV-G-RSV-Rev (RDB04393)
pCAG-HIVgp (RDB04394) |
