論文 - 詳細
| RRC ID | 67122 |
|---|---|
| 著者 | Miyazaki S, Tashiro F, Tsuchiya T, Sasaki K, Miyazaki JI. |
| タイトル | Establishment of a long-term stable β-cell line and its application to analyze the effect of Gcg expression on insulin secretion. |
| ジャーナル | Sci Rep |
| Abstract |
A pancreatic β-cell line MIN6 was previously established in our lab from an insulinoma developed in an IT6 transgenic mouse expressing the SV40 T antigen in β-cells. This cell line has been widely used for in vitro analysis of β-cell function, but tends to lose the mature β-cell features, including glucose-stimulated insulin secretion (GSIS), in long-term culture. The aim of this study was to develop a stable β-cell line that retains the characteristics of mature β-cells. Considering that mice derived from a cross between C3H and C57BL/6 strains are known to exhibit higher insulin secretory capacity than C57BL/6 mice, an IT6 male mouse of this hybrid background was used to isolate insulinomas, which were independently cultured. After 7 months of continuous culturing, we obtained the MIN6-CB4 β-cell line, which stably maintains its GSIS. It has been noted that β-cell lines express the glucagon (Gcg) gene at certain levels. MIN6-CB4 cells were utilized to assess the effects of differential Gcg expression on β-cell function. Our data show the functional importance of Gcg expression and resulting basal activation of the GLP-1 receptor in β-cells. MIN6-CB4 cells can serve as an invaluable tool for studying the regulatory mechanisms of insulin secretion, such as the GLP-1/cAMP signaling, in β-cells. |
| 巻・号 | 11(1) |
| ページ | 477 |
| 公開日 | 2021-1-12 |
| DOI | 10.1038/s41598-020-79992-7 |
| PII | 10.1038/s41598-020-79992-7 |
| PMID | 33436850 |
| PMC | PMC7804151 |
| MeSH | Animals Female Glucagon / physiology* Glucagon-Like Peptide-1 Receptor / metabolism* Insulin Secretion* Insulin-Secreting Cells / cytology Insulin-Secreting Cells / metabolism* Insulinoma / metabolism* Insulinoma / pathology Male Mice Mice, Inbred C3H Mice, Inbred C57BL Mice, Knockout Pancreatic Neoplasms / metabolism* Pancreatic Neoplasms / pathology |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | CS-CDF-CG-PRE (RDB04379) pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394) |