論文 - 詳細
| RRC ID | 67133 |
|---|---|
| 著者 | Kawakatsu K, Maemura M, Seta Y, Nishikata T. |
| タイトル | Involvement of Annexin A2 in Serum-MAF Dependent Phagocytic Activation of Macrophages. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:Serum-derived macrophage activating factor (serum-MAF) is expected to have adjuvant effects through rapid phagocytic activation, which depends on F-actin accumulation in multi-layered membrane ruffles induced within 5 min after serum-MAF addition. This study aimed to elucidate the importance of annexin A2, which is a multifunctional Ca2+-binding protein related to cytoskeletal membrane dynamics, in serum-MAF signalling. MATERIALS AND METHODS:Annexin A2 and F-actin localizations were analyzed via immunostaining and confocal microscopy. Using EGTA as chelator, the role of Ca2+ in serum-MAF signalling was examined. RESULTS:Annexin A2 was found to translocate from the cytosol to the cell cortex within 30 s of serum-MAF stimulation. Ca2+ chelation inhibited the translocation of annexin A2, frill-like structure formation, and phagocytic activation by serum-MAF. CONCLUSION:Annexin A2 and Ca2+ were responsible for the rapid phagocytic activation by serum-MAF. This study provides an understanding of phagocytic activation in macrophages, which could be beneficial for cancer immunotherapy. |
| 巻・号 | 41(8) |
| ページ | 4089-4092 |
| 公開日 | 2021-8-1 |
| DOI | 10.21873/anticanres.15211 |
| PII | 41/8/4089 |
| PMID | 34281879 |
| MeSH | Actins / metabolism Annexin A2 / metabolism* Humans Macrophage Activation* Macrophage-Activating Factors / blood* Macrophages / physiology* Phagocytosis THP-1 Cells |
| IF | 1.994 |
| リソース情報 | |
| ヒト・動物細胞 | THP-1(RCB1189) |