| RRC ID |
67187
|
| 著者 |
Murakami M, Yoshihara K, Shimbara S, Lambeau G, Singer A, Gelb MH, Sawada M, Inagaki N, Nagai H, Kudo I.
|
| タイトル |
Arachidonate release and eicosanoid generation by group IIE phospholipase A(2).
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
The heparin-binding group II subfamily of secretory phospholipase A(2)s (sPLA(2)s), such as sPLA(2)-IIA and -IID, augments stimulus-induced arachidonic acid (AA) release through the cellular heparan sulfate proteoglycan (HSPG)-dependent pathway when transfected into HEK293 cells. Here we show that the closest homolog, sPLA(2)-IIE, also promotes stimulus-induced AA release and prostaglandin (PG) production similar to those elicited by HSPG-dependent sPLA(2)s. Confocal laser microscopic analysis demonstrates the location of sPLA(2)-IIE in cytoplasmic punctate compartments. sPLA(2)-IIE also enhances leukotriene (LT) production and granule exocytosis by RBL-2H3 mastocytoma cells. Expression of sPLA(2)-IIE was highly upregulated in mice injected with lipopolysaccharide (LPS) and in mice with experimental atopic dermatitis. These observations suggest that this enzyme plays a role in the inflammatory process, as proposed for other group II subfamily sPLA(2)s.
|
| 巻・号 |
292(3)
|
| ページ |
689-96
|
| 公開日 |
2002-4-5
|
| DOI |
10.1006/bbrc.2002.6716
|
| PII |
S0006291X02967161
|
| PMID |
11922621
|
| MeSH |
Animals
Anticoagulants / pharmacology
Arachidonic Acid / metabolism*
Cell Line
Eicosanoids / metabolism*
Gene Expression Regulation
Group II Phospholipases A2
Heparin / pharmacology
Humans
Inflammation / physiopathology*
Isoenzymes / metabolism
Mast Cells / metabolism
Mice
Microscopy, Confocal
Phospholipases A / genetics
Phospholipases A / metabolism*
Protein Binding
|
| IF |
2.985
|
| リソース情報 |
| ヒト・動物細胞 |
RBL 2H3(RCB2782) |
