| RRC ID |
67329
|
| 著者 |
Banerjee D, Fernandez HR, Patil PB, Premaratne P, Quiding-Järbrink M, Lindén SK.
|
| タイトル |
Epithelial MUC1 promotes cell migration, reduces apoptosis and affects levels of mucosal modulators during acetylsalicylic acid (aspirin)-induced gastropathy.
|
| ジャーナル |
Biochem J
|
| Abstract |
MUC1 is a transmembrane mucin highly expressed in the stomach. Although extensive research has uncovered many of its roles in cancer, knowledge about the functions of MUC1 in normal tissues is limited. In the present study, we showed that acetylsalicylic acid (ASA; aspirin) up-regulated MUC1/Muc1 expression in the gastric mucosa of humans and wild-type (WT) mice. ASA induced mucosal injury in all mice to a similar extent; however, WT animals and those chimaeras with Muc1 on the epithelia recovered faster than Muc1-knockout (KO) mice and chimaeras carrying Muc1 on haemopoietic but not epithelial cells. MUC1 enhanced proliferation and migration of the human gastric cell line MKN-7 and increased resistance to apoptosis. The repeated treatment regime used caused a reduction in cyclo-oxygenase-1 (Cox-1) expression, though WT animals returned faster towards pre-treatment levels and had increased Cox-2 and vascular endothelial growth factor levels during recovery. Thus we found that epithelial Muc1 is more important for the healing process than haemopoietic Muc1 and Muc1/MUC1 facilitates wound healing by enhancing cell migration and proliferation, protecting against apoptosis and mediating expression of mucosal modulators. Thus MUC1 plays essential roles during wound healing and development of treatment modalities targeting enhanced expression of MUC1 may be beneficial to treat mucosal wounds.
|
| 巻・号 |
465(3)
|
| ページ |
423-31
|
| 公開日 |
2015-2-1
|
| DOI |
10.1042/BJ20140617
|
| PII |
BJ20140617
|
| PMID |
25387004
|
| MeSH |
Animals
Apoptosis / drug effects
Apoptosis / physiology*
Aspirin / toxicity*
Cell Movement / drug effects
Cell Movement / physiology*
Epithelial Cells / drug effects
Epithelial Cells / metabolism*
Epithelial Cells / pathology
Gastric Mucosa / drug effects*
Gastric Mucosa / metabolism*
Gastric Mucosa / pathology
Humans
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Mucin-1 / biosynthesis*
|
| IF |
4.097
|
| リソース情報 |
| ヒト・動物細胞 |
MKN7(RCB0999) |
