論文 - 詳細
| RRC ID | 67343 |
|---|---|
| 著者 | Maeda O, Kondo M, Fujita T, Usami N, Fukui T, Shimokata K, Ando T, Goto H, Sekido Y. |
| タイトル | Enhancement of GLI1-transcriptional activity by beta-catenin in human cancer cells. |
| ジャーナル | Oncol Rep |
| Abstract |
The Hedgehog (Hh) signaling pathway and the Wnt signaling pathway are known to play important roles in carcinogenesis and the progression of various human malignant tumors. Although a relationship between these two pathways has recently been reported, the mechanism by which beta-catenin, one of the key molecules of the Wnt signaling pathway, influences the Hh pathway has not yet been revealed in detail. To clarify the role of beta-catenin in relation to the Hh signaling pathway, we transfected GLI1 and beta-catenin expression constructs into human malignant cells, including stomach, colon, and lung cancers, and evaluated the luciferase activity of GLI-responsive reporter constructs. While exogenous GLI1 increased the luciferase activity, exogenous beta-catenin also enhanced the activity under overexpression of GLI1. However, co-transfection with T-cell factor (TCF)-4 or lymphocyte enhancer factor (LEF)-1 did not influence the activity, indicating that the enhancement of beta-catenin in relation to the Hh signaling pathway is not TCF/LEF-dependent. Our results suggest that beta-catenin might be involved in the Hh signaling pathway via enhancement of the transcriptional activity of GLI. |
| 巻・号 | 16(1) |
| ページ | 91-6 |
| 公開日 | 2006-7-1 |
| PMID | 16786128 |
| MeSH | Cell Line, Tumor Gene Expression Regulation, Neoplastic* Genes, Reporter Hedgehog Proteins Humans Luciferases / metabolism Lymphoid Enhancer-Binding Factor 1 / metabolism Oncogene Proteins / genetics* Oncogene Proteins / physiology* Signal Transduction TCF Transcription Factors / metabolism Trans-Activators / genetics* Trans-Activators / metabolism Trans-Activators / physiology* Transcription, Genetic* Wnt Proteins / metabolism Zinc Finger Protein GLI1 beta Catenin / metabolism* |
| IF | 3.417 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | HGC-27(RCB0500) CW-2(RCB0778) COLO-320(RCB1193) |