Reference - Detail
| RRC ID | 6740 |
|---|---|
| Author | Shimada N, Kawata T. |
| Title | Evidence that noncoding RNA dutA is a multicopy suppressor of Dictyostelium discoideum STAT protein Dd-STATa. |
| Journal | Eukaryot Cell |
| Abstract |
Dd-STATa, a Dictyostelium discoideum homologue of metazoan STAT transcription factors, is necessary for culmination. We created a mutant strain with partial Dd-STATa activity and used it to screen for unlinked suppressor genes. We screened approximately 450,000 clones from a slug-stage cDNA library for their ability to rescue the culmination defect when overexpressed. There were 12 multicopy suppressors of Dd-STATa, of which 4 encoded segments of a known noncoding RNA, dutA. Expression of dutA is specific to the pstA zone, the region where Dd-STATa is activated. In suppressed strains the expression patterns of several putative Dd-STATa target genes become similar to the wild-type strain. In addition, the amount of the tyrosine-phosphorylated form of Dd-STATa is significantly increased in the suppressed strain. These results indicate that partial copies of dutA may act upstream of Dd-STATa to regulate tyrosine phosphorylation by an unknown mechanism. |
| Volume | 6(6) |
| Pages | 1030-40 |
| Published | 2007-6-1 |
| DOI | 10.1128/EC.00035-07 |
| PII | EC.00035-07 |
| PMID | 17435008 |
| PMC | PMC1951520 |
| MeSH | Animals Dictyostelium* / genetics Dictyostelium* / metabolism Genes, Protozoan* MicroRNAs / genetics MicroRNAs / metabolism Phosphorylation Protozoan Proteins / genetics Protozoan Proteins / metabolism* RNA Precursors / genetics RNA Precursors / metabolism RNA, Small Interfering / genetics RNA, Small Interfering / metabolism RNA, Untranslated / genetics RNA, Untranslated / metabolism* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Repressor Proteins / genetics Repressor Proteins / metabolism* STAT Transcription Factors / genetics STAT Transcription Factors / metabolism* Tyrosine / metabolism |
| IF | 2.992 |
| Times Cited | 12 |
| WOS Category | MYCOLOGY MICROBIOLOGY |
| Resource | |
| Cellular slime molds | G24894 |