論文 - 詳細
| RRC ID | 67419 |
|---|---|
| 著者 | Asanuma D, Sakabe M, Kamiya M, Yamamoto K, Hiratake J, Ogawa M, Kosaka N, Choyke PL, Nagano T, Kobayashi H, Urano Y. |
| タイトル | Sensitive β-galactosidase-targeting fluorescence probe for visualizing small peritoneal metastatic tumours in vivo. |
| ジャーナル | Nat Commun |
| Abstract |
Fluorescence-guided diagnostics is one of the most promising approaches for facile detection of cancer in situ. Here we focus on β-galactosidase, which is overexpressed in primary ovarian cancers, as a molecular target for visualizing peritoneal metastases from ovarian cancers. As existing fluorescence probes are unsuitable, we have designed membrane-permeable HMRef-βGal, in which the optimized intramolecular spirocyclic function affords >1,400-fold fluorescence enhancement on activation. We confirm that HMRef-βGal sensitively detects intracellular β-galactosidase activity in several ovarian cancer lines. In vivo, this probe visualizes metastases as small as <1 mm in diameter in seven mouse models of disseminated human peritoneal ovarian cancer (SHIN3, SKOV3, OVK18, OVCAR3, OVCAR4, OVCAR5 and OVCAR8). Because of its high brightness, real-time detection of metastases with the naked eye is possible. Endoscopic fluorescence detection of metastases is also demonstrated. The results clearly indicate preclinical potential value of the probe for fluorescence-guided diagnosis of peritoneal metastases from ovarian cancers. |
| 巻・号 | 6 |
| ページ | 6463 |
| 公開日 | 2015-3-13 |
| DOI | 10.1038/ncomms7463 |
| PII | ncomms7463 |
| PMID | 25765713 |
| PMC | PMC4382686 |
| MeSH | Animals Disease Models, Animal Endoscopy Female Fluorescent Dyes / chemistry* Gene Expression Regulation, Neoplastic Humans Mice Mice, Inbred BALB C Microscopy, Fluorescence Neoplasm Metastasis Ovarian Neoplasms / diagnosis* Ovarian Neoplasms / pathology Peritoneal Neoplasms / diagnosis* Peritoneal Neoplasms / secondary beta-Galactosidase / metabolism* |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 44 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 6.0 |
| リソース情報 | |
| ヒト・動物細胞 | NIH:OVCAR-3(RCB2135) OVK18(RCB1903) |