RRC ID 6743
著者 Kalavrizioti D, Vourekas A, Drainas D.
タイトル DRpp20 and DRpp40: Two protein subunits involved in Dictyostelium discoideum ribonuclease P holoenzyme assembly.
ジャーナル Gene
Abstract Ribonuclease P is an essential enzyme that matures the 5' ends of all primary tRNA transcripts. RNase P enzymes contain a similar in size RNA subunit which is absolutely required for catalysis. The holoenzyme from Dictyostelium discoideum possesses an essential for activity RNA subunit but the exact protein composition is still under investigation. Bioinformatic analysis of D. discoideum sequencing data returned seven ORFs homologous to previously characterized RNase P protein subunits from human. In the present study, DRpp20 and DRpp40 were cloned and characterized. These proteins apart from the noted similarity possess idiosyncratic regions. Immunobiochemical analysis presented herein indicates their direct involvement in the formation of the ribonucleoprotein complex of D. discoideum RNase P holoenzyme.
巻・号 400(1-2)
ページ 52-9
公開日 2007-7-6
DOI 10.1016/j.gene.2007.05.020
PII S0378-1119(07)00296-X
PMID 17611045
MeSH Amino Acid Sequence Animals Base Sequence Cloning, Molecular Dictyostelium / enzymology* Holoenzymes / metabolism* Molecular Sequence Data Protein Subunits / metabolism* Protozoan Proteins / metabolism* Ribonuclease P / metabolism* Sequence Homology, Amino Acid
IF 2.415
引用数 2
細胞性粘菌 G02201 G21765