論文 - 詳細
| RRC ID | 67464 |
|---|---|
| 著者 | Honda S, Loher P, Shigematsu M, Palazzo JP, Suzuki R, Imoto I, Rigoutsos I, Kirino Y. |
| タイトル | Sex hormone-dependent tRNA halves enhance cell proliferation in breast and prostate cancers. |
| ジャーナル | Proc Natl Acad Sci U S A |
| Abstract |
Sex hormones and their receptors play critical roles in the development and progression of the breast and prostate cancers. Here we report that a novel type of transfer RNA (tRNA)-derived small RNA, termed Sex HOrmone-dependent TRNA-derived RNAs (SHOT-RNAs), are specifically and abundantly expressed in estrogen receptor (ER)-positive breast cancer and androgen receptor (AR)-positive prostate cancer cell lines. SHOT-RNAs are not abundantly present in ER(-) breast cancer, AR(-) prostate cancer, or other examined cancer cell lines from other tissues. ER-dependent accumulation of SHOT-RNAs is not limited to a cell culture system, but it also occurs in luminal-type breast cancer patient tissues. SHOT-RNAs are produced from aminoacylated mature tRNAs by angiogenin-mediated anticodon cleavage, which is promoted by sex hormones and their receptors. Resultant 5'- and 3'-SHOT-RNAs, corresponding to 5'- and 3'-tRNA halves, bear a cyclic phosphate (cP) and an amino acid at the 3'-end, respectively. By devising a "cP-RNA-seq" method that is able to exclusively amplify and sequence cP-containing RNAs, we identified the complete repertoire of 5'-SHOT-RNAs. Furthermore, 5'-SHOT-RNA, but not 3'-SHOT-RNA, has significant functional involvement in cell proliferation. These results have unveiled a novel tRNA-engaged pathway in tumorigenesis of hormone-dependent cancers and implicate SHOT-RNAs as potential candidates for biomarkers and therapeutic targets. |
| 巻・号 | 112(29) |
| ページ | E3816-25 |
| 公開日 | 2015-7-21 |
| DOI | 10.1073/pnas.1510077112 |
| PII | 1510077112 |
| PMID | 26124144 |
| PMC | PMC4517238 |
| MeSH | Amino Acids / metabolism Animals Base Sequence Bombyx Breast Neoplasms / metabolism* Breast Neoplasms / pathology* Cell Line, Tumor Cell Proliferation Epithelial Cells / metabolism Female Gene Knockdown Techniques Gonadal Steroid Hormones / metabolism* Humans Hydroxylation Male Models, Biological Molecular Sequence Data Phosphates Prostatic Neoplasms / metabolism* Prostatic Neoplasms / pathology* RNA, Transfer / chemistry RNA, Transfer / genetics RNA, Transfer / metabolism* Real-Time Polymerase Chain Reaction Receptors, Androgen / metabolism Receptors, Estrogen / metabolism Ribonuclease, Pancreatic / metabolism Sequence Analysis, RNA |
| IF | 9.412 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 31 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-3(RCB2145) PANC-1(RCB2095) MIA Paca2(RCB2094) HuH-7(RCB1942) Hep G2(RCB1886) LNCap.FGC(RCB2144) DU145(RCB2143) KLM-1(RCB2138) Kato III(RCB2088) NUGC-4(RCB1939) LK-2(RCB1970) MS-1(RCB0725) SH-10-TC(RCB1940) COLO-320(RCB1193) HGC-27(RCB0500) TE-6(RCB1950) TE-5(RCB1949) TE-14(RCB2101) TE-11(RCB2100) TE-8(RCB2098) |