RRC ID 67513
Author Kowada T, Watanabe T, Liu R, Mizukami S.
Title Protocol for synthesis and use of a turn-on fluorescent probe for quantifying labile Zn2+ in the Golgi apparatus in live cells.
Journal STAR Protoc
Abstract Quantitative analysis using a turn-on fluorescent probe is inherently difficult due to the dependency of the fluorescence intensity on the probe concentration. To overcome this limitation, we developed an in situ quantification method using a turn-on fluorescent probe and a standard fluorophore, which are colocalized by protein tag technology. This protocol describes the synthesis of a Zn2+ probe, named ZnDA-1H, and the procedure to quantify the labile Zn2+ concentration in the Golgi of live HeLa cells by confocal fluorescence microscopy. For complete details on the use and execution of this protocol, please refer to Kowada et al. (2020).
Volume 2(2)
Pages 100395
Published 2021-6-18
DOI 10.1016/j.xpro.2021.100395
PII S2666-1667(21)00102-7
PMID 33796872
Resource
Human and Animal Cells HeLa(RCB0007)