論文 - 詳細
| RRC ID | 67515 |
|---|---|
| 著者 | Arai M, Komori H, Fujita D, Tamai I. |
| タイトル | Uptake Pathway of Apple-derived Nanoparticle by Intestinal Cells to Deliver its Cargo. |
| ジャーナル | Pharm Res |
| Abstract |
PURPOSE:Food-derived nanoparticles exert cytoprotective effects on intestinal cells by delivering their cargo, which includes macromolecules such as microRNAs and proteins, as well as low-molecular weight compounds. We previously reported that apple-derived nanoparticles (APNPs) downregulate the expression of human intestinal transporter OATP2B1/SLCO2B1 mRNA. To verify the involvement of the cargo of APNPs in affecting the expression of transporters, we characterized the uptake mechanism of APNPs in intestinal cells. METHODS:The uptake of fluorescent PKH26-labeled APNPs (PKH-APNPs) into Caco-2, LS180, and HT-29MTX cells was evaluated by confocal microscopy and flow cytometry. RESULTS:The uptake of PKH-APNPs was prevented in the presence of clathrin-dependent endocytosis inhibitors, chlorpromazine and Pitstop2. Furthermore, PKH-APNPs were incorporated by the HT29-MTX cells, despite the disturbance of the mucus layer. Additionally, the decrease in SLCO2B1 mRNA by APNPs was reversed by Pitstop 2 in Caco-2 cells, indicating that APNPs decrease SLCO2B1 by being incorporated via clathrin-dependent endocytosis. CONCLUSIONS:We demonstrated that clathrin-dependent endocytosis was mainly involved in the uptake of APNPs by intestinal cells, and that the cargo in the APNPs downregulate the mRNA expression of SLCO2B1. Therefore, APNPs could be a useful tool to deliver large molecules such as microRNAs to intestinal cells. |
| 巻・号 | 38(3) |
| ページ | 523-530 |
| 公開日 | 2021-3-1 |
| DOI | 10.1007/s11095-021-03018-8 |
| PII | 10.1007/s11095-021-03018-8 |
| PMID | 33723795 |
| MeSH | Biological Transport Caco-2 Cells Clathrin / metabolism Endocytosis Fluorescent Dyes / chemistry Gene Expression Regulation / drug effects HT29 Cells Humans Intestines / cytology Intestines / pathology* Malus / chemistry* Nanoparticles / chemistry* Nanoparticles / metabolism* Optical Imaging Organic Anion Transporters / genetics Organic Anion Transporters / metabolism RNA, Messenger / metabolism Signal Transduction Tissue Distribution |
| IF | 3.242 |
| リソース情報 | |
| ヒト・動物細胞 | CACO-2(RCB0988) |