| RRC ID |
67525
|
| Author |
Ozeki K, Kato M, Sakurai Y, Ishigai M, Kudo T, Ito K.
|
| Title |
Evaluation of the appropriate time range for estimating the apparent permeability coefficient (P(app)) in a transcellular transport study.
|
| Journal |
Int J Pharm
|
| Abstract |
In a transcellular transport study, the apparent permeability coefficient (Papp) of a compound is evaluated using the range by which the amount of compound accumulated on the receiver side is assumed to be proportional to time. However, the time profile of the concentration of the compound in receiver (C3) often shows a lag time before reaching the linear range and later changes from linear to steady state. In this study, the linear range needed to calculate Papp in the C3-time profile was evaluated by a 3-compartment model. C3 was described by an equation with two steady states (C3=A3(1-e(-αt))+B3(1-e(-βt)), α>β), and by a simple approximate line (C3=A3-A3×αt) in the time range of 3/α
|
| Volume |
495(2)
|
| Pages |
963-71
|
| Published |
2015-11-30
|
| DOI |
10.1016/j.ijpharm.2015.09.035
|
| PII |
S0378-5173(15)30233-7
|
| PMID |
26387619
|
| MeSH |
Caco-2 Cells
Humans
Intestinal Absorption
Kinetics
Models, Biological*
Permeability
Time Factors
Transcytosis*
|
| IF |
4.845
|
| Resource |
| Human and Animal Cells |
CACO-2(RCB0988) |
