Reference - Detail
| RRC ID | 67544 |
|---|---|
| Author | Harada K, Baba Y, Ishimoto T, Kosumi K, Tokunaga R, Izumi D, Ohuchi M, Nakamura K, Kiyozumi Y, Kurashige J, Iwagami S, Miyamoto Y, Sakamoto Y, Yoshida N, Oki E, Watanabe M, Baba H. |
| Title | Suppressor microRNA-145 Is Epigenetically Regulated by Promoter Hypermethylation in Esophageal Squamous Cell Carcinoma. |
| Journal | Anticancer Res |
| Abstract |
BACKGROUND/AIM:DNA methylation is a common epigenetic change in cancer. However, microRNA (miRNA) regulation by epigenetic alteration, especially CpG island hypermethylation, remains poorly understood in esophageal squamous cell carcinoma (ESCC). MATERIALS AND METHODS:miRNAs which were up-regulated after de-methylation with 5-aza-2'-deoxycytidine (5-AZA) were analyzed using the Human miFinder 384HC miScript miRNA PCR Array. The DNA methylation level was evaluated by bisulfite-pyrosequencing assay. RESULTS:In two of the cell lines, 20 miRNAs, including miR-145-5p, were found to be up-regulated by more than three-fold after 5-AZA treatment. The miRNA-145 promoter was significantly more hypermethylated in the cancer tissues than in matched normal adjacent esophageal epithelial mucosa (p=0.0042; paired t-test). Moreover, the miRNA-145-5p expression levels were significantly lower in cancerous tissues (p=0.0024). CONCLUSION:miRNA-145 expression in ESCC seems to be regulated by hypermethylation of the miRNA-145 promoter region. |
| Volume | 35(9) |
| Pages | 4617-24 |
| Published | 2015-9-1 |
| PII | 35/9/4617 |
| PMID | 26254350 |
| MeSH | Aged Carcinoma, Squamous Cell / genetics* CpG Islands DNA Methylation / genetics* Disease-Free Survival Epigenesis, Genetic* Esophageal Neoplasms / genetics* Esophageal Squamous Cell Carcinoma Female Gene Expression Regulation, Neoplastic Humans Kaplan-Meier Estimate Male MicroRNAs / genetics MicroRNAs / metabolism* Middle Aged Mucous Membrane / metabolism Mucous Membrane / pathology Oligonucleotide Array Sequence Analysis Promoter Regions, Genetic* |
| IF | 1.994 |
| Resource | |
| Human and Animal Cells | TE-1(RCB1894) TE-4(RCB2097) TE-6(RCB1950) TE-8(RCB2098) TE-9(RCB1988) TE-10(RCB2099) TE-11(RCB2100) TE-14(RCB2101) TE-15(RCB1951) |