| RRC ID |
67558
|
| 著者 |
Miyata K, Suzuki T, Hayashi S, Miyashita S, Ohyama T, Niwa K, Watanabe T, Sagane Y.
|
| タイトル |
Hemagglutinin gene shuffling among Clostridium botulinum serotypes C and D yields distinct sugar recognition of the botulinum toxin complex.
|
| ジャーナル |
Pathog Dis
|
| Abstract |
Clostridium botulinum strains produce a large-sized toxin complex (TC) that is composed of botulinum neurotoxin (BoNT), non-toxic non-hemagglutinin and three different hemagglutinins (HA-70, HA-33 and HA-17). HA components enhance toxin delivery across the intestinal cell wall in a sugar chain-dependent manner. Here we characterized the sugar recognition of serotype D strain 1873 (D-1873) botulinum L-TC. Most L-TCs produced by serotype C and D strains bind to cells via interactions between HA-33 and cell surface sialo-oligosaccharides. However, like the previously reported L-TC produced by serotype C strain Yoichi (C-Yoichi), D-1873 L-TC binds only to cells that have been treated with neuraminidase, indicating that they recognize asialo-oligosaccharides. The D-1873 HA-33 amino acid sequence is similar to that of C-Yoichi, but had lower similarity to the majority of serotype C and D HA-33s. A comparison of TC component primary structures for 12 serotype C and D strains suggested that at least three types of HA-33 genes exist, and these are shuffled among the serotype C and D strains independently of BoNT serotype. This shuffling produces the distinct sugar recognition of serotype C and D botulinum TCs.
|
| 巻・号 |
73(7)
|
| 公開日 |
2015-10-1
|
| DOI |
10.1093/femspd/ftv054
|
| PII |
ftv054
|
| PMID |
26223883
|
| MeSH |
Animals
Botulinum Toxins / metabolism*
Cell Line
Clostridium botulinum / genetics*
DNA Shuffling*
Epithelial Cells / metabolism
Erythrocytes
Hemagglutination Tests
Hemagglutinins / genetics*
Hemagglutinins / metabolism*
Horses
Oligosaccharides / metabolism*
Protein Binding
Rats
Substrate Specificity
|
| IF |
2.166
|
| リソース情報 |
| ヒト・動物細胞 |
IEC 6(RCB0993) |
