RRC ID 67573
Author Kawami M, Miyamoto M, Yumoto R, Takano M.
Title Methotrexate influx via folate transporters into alveolar epithelial cell line A549.
Journal Drug Metab Pharmacokinet
Abstract Methotrexate (MTX), a drug used for the treatment of certain cancers as well as rheumatoid arthritis, sometimes induces serious interstitial lung injury. Although lung toxicity of MTX is related to its accumulation, the information concerning MTX transport in the lungs is lacking. In this study, we investigated the mechanisms underlying MTX influx into human alveolar epithelial cell line A549. MTX influx into A549 cells was time-, pH-, and temperature-dependent and showed saturation kinetics. The influx was inhibited by folic acid with IC50 values of 256.1 μM at pH 7.4 and 1.6 μM at pH 5.5, indicating that the mechanisms underlying MTX influx would be different at these pHs. We then examined the role of two folate transporters in MTX influx, reduced folate carrier (RFC) and proton-coupled folate transporter (PCFT). The expression of RFC and PCFT mRNAs in A549 cells was confirmed by reverse transcription polymerase chain reaction. In addition, MTX influx was inhibited by thiamine monophosphate, an RFC inhibitor, at pH 7.4, and by sulfasalazine, a PCFT inhibitor, at pH 5.5. These results indicated that RFC and PCFT are predominantly involved in MTX influx into A549 cells at pH 7.4 and pH 5.5, respectively.
Volume 30(4)
Pages 276-81
Published 2015-8-1
DOI 10.1016/j.dmpk.2015.04.005
PII S1347-4367(15)00027-0
PMID 26190800
MeSH Biological Transport / drug effects Biological Transport / physiology Carrier Proteins / metabolism Cell Line, Tumor Epithelial Cells / drug effects Epithelial Cells / metabolism* Folic Acid / metabolism* Folic Acid Transporters / metabolism* Humans Hydrogen-Ion Concentration Kinetics Methotrexate / metabolism* Proton-Coupled Folate Transporter / metabolism Pulmonary Alveoli / drug effects Pulmonary Alveoli / metabolism* Sulfasalazine / pharmacology
IF 2.772
Human and Animal Cells A549