| RRC ID |
6763
|
| Author |
Roybon L, Ma Z, Asztely F, Fosum A, Jacobsen SE, Brundin P, Li JY.
|
| Title |
Failure of transdifferentiation of adult hematopoietic stem cells into neurons.
|
| Journal |
Stem Cells
|
| Abstract |
Previous studies of bone marrow-derived stem cell transdifferentiation into neurons have not involved purified cell populations and determined their exact phenotype prior to differentiation. The present study investigates whether highly purified mouse adult hematopoietic stem cells (HSCs), characterized by lineage marker depletion and expression of the cell surface markers Sca1 and c-Kit (Lin(-) Sca1(+) c-Kit(+) [LSK]), can be stimulated to adopt a neuronal fate. When the HSC(LSK) cells were cultured in vitro in neuronal differentiation medium supplemented with retinoic acid, 50% of the cells expressed the neural progenitor marker nestin and no cells had become postmitotic. Electrophysiological recordings on neuron-like cells showed that these cells were incapable of generating action potentials. When the HSC(LSK) cells either were grown in vitro together with neural precursor cells or were transplanted into the striatum or cerebellum of wild-type mouse, they either differentiated into Iba1-immunopositive macrophage/microglia or died. In conclusion, we demonstrate that adult HSC(LSK) cells do not have the capacity to leave the hematopoietic lineage and differentiate into neurons.
|
| Volume |
24(6)
|
| Pages |
1594-604
|
| Published |
2006-6-1
|
| DOI |
10.1634/stemcells.2005-0548
|
| PII |
2005-0548
|
| PMID |
16556707
|
| MeSH |
Animals
Biomarkers / metabolism
Brain / cytology
Cell Cycle
Cell Differentiation
Cell Proliferation
Cell Separation
Electrophysiology
Green Fluorescent Proteins / genetics
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells / classification
Hematopoietic Stem Cells / cytology*
Hematopoietic Stem Cells / metabolism
In Vitro Techniques
Intermediate Filament Proteins / metabolism
Male
Mice
Mice, Transgenic
Nerve Tissue Proteins / metabolism
Nestin
Neurons / cytology*
Tubulin / metabolism
|
| IF |
6.022
|
| Times Cited |
45
|
|
WOS Category
|
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
HEMATOLOGY
ONCOLOGY
CELL BIOLOGY
CELL & TISSUE ENGINEERING
|
| Resource |
| Human and Animal Cells |
MC3T3-G2/PA6(RCB1127) |
