| RRC ID |
6764
|
| Author |
Ikeda T, Saito T, Ushita M, Yano F, Kan A, Itaka K, Moro T, Nakamura K, Kawaguchi H, Chung UI.
|
| Title |
Identification and characterization of the human SOX6 promoter.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
The present study attempted to identify and characterize the embryonic promoter of Sox6, a determinant regulator of chondrogenic differentiation. A common transcription start region for human and mouse Sox6 was initially identified, which contained a highly conserved sequence, A-box. Tandem repeats of A-box had a strong transcriptional activity both at the basal level and in response to Sox9. Cells carrying the 4xA-box-DsRed2 reporter fluoresced only upon chondrogenic differentiation. The 46-bp core enhancer region (CES6) was then identified in the 3' half of A-box, within which a C/EBP-binding motif was identified. Overexpressed C/EBPbeta activated the Sox6 promoter, and mutant 4xCES6 constructs lacking the C/EBP motif lost their basal activity. CES6 and nuclear extracts formed a specific complex, which was supershifted by anti-C/EBPbeta antibody, and in vitro translated C/EBPbeta specifically bound to CES6. Thus, we successfully identified the Sox6 promoter and its core enhancer and characterized the interactions with regulatory transcription factors.
|
| Volume |
357(2)
|
| Pages |
383-90
|
| Published |
2007-6-1
|
| DOI |
10.1016/j.bbrc.2007.03.133
|
| PII |
S0006-291X(07)00625-0
|
| PMID |
17433257
|
| MeSH |
Animals
Base Sequence
Chondrogenesis / genetics*
DNA-Binding Proteins / genetics*
High Mobility Group Proteins / genetics*
Humans
Mice
Molecular Sequence Data
Promoter Regions, Genetic / genetics*
Regulatory Sequences, Nucleic Acid / genetics*
SOXD Transcription Factors
Transcription Factors / genetics*
|
| IF |
2.985
|
| Times Cited |
10
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
HuH-7(RCB1366) |
