論文 - 詳細
| RRC ID | 67693 |
|---|---|
| 著者 | Hirai H, Kamio N, Huang G, Matsusue A, Ogino S, Kimura N, Satake S, Ashihara E, Imanishi J, Tenen DG, Maekawa T. |
| タイトル | Cyclic AMP responsive element binding proteins are involved in 'emergency' granulopoiesis through the upregulation of CCAAT/enhancer binding protein β. |
| ジャーナル | PLoS One |
| Abstract |
In contrast to the definitive role of the transcription factor, CCAAT/Enhancer binding protein α (C/EBPα), in steady-state granulopoiesis, previous findings have suggested that granulopoiesis during emergency situations, such as infection, is dependent on C/EBPβ. In this study, a novel lentivirus-based reporter system was developed to elucidate the molecular switch required for C/EBPβ-dependency. The results demonstrated that two cyclic AMP responsive elements (CREs) in the proximal promoter region of C/EBPβ were involved in the positive regulation of C/EBPβ transcription during granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced differentiation of bone marrow cells. In addition, the transcripts of CRE binding (CREB) family proteins were readily detected in hematopoietic stem/progenitor cells. CREB was upregulated, phosphorylated and bound to the CREs in response to GM-CSF stimulation. Retroviral transduction of a dominant negative CREB mutant reduced C/EBPβ mRNA levels and significantly impaired the proliferation/differentiation of granulocyte precursors, while a constitutively active form of CREB facilitated C/EBPβ transcription. These data suggest that CREB proteins are involved in the regulation of granulopoiesis via C/EBPβ upregulation. |
| 巻・号 | 8(1) |
| ページ | e54862 |
| 公開日 | 2013-1-1 |
| DOI | 10.1371/journal.pone.0054862 |
| PII | PONE-D-11-25889 |
| PMID | 23382991 |
| PMC | PMC3559830 |
| MeSH | Animals CCAAT-Enhancer-Binding Protein-beta / genetics* CCAAT-Enhancer-Binding Protein-beta / metabolism Cell Line Cyclic AMP Response Element-Binding Protein / metabolism* Gene Expression Regulation / drug effects Gene Order Genes, Reporter Genetic Vectors / genetics Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology Granulocytes / metabolism* Humans Lentivirus / genetics Mice Myelopoiesis / physiology* Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Signal Transduction Transcriptional Activation / drug effects |
| IF | 2.74 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | CS-CDF-EG-PRE (RDB04380) |