RRC ID 67701
著者 Takahashi A, Ishii G, Neri S, Yoshida T, Hashimoto H, Suzuki S, Umemura S, Matsumoto S, Yoh K, Niho S, Goto K, Ohmatsu H, Nagai K, Gemma A, Ohe Y, Ochiai A.
タイトル Podoplanin-expressing cancer-associated fibroblasts inhibit small cell lung cancer growth.
ジャーナル Oncotarget
Abstract Cancer-associated fibroblasts (CAFs) expressing podoplanin (PDPN) are a favorable prognosticator in surgically resected small cell lung cancer (SCLC). Here we explore whether CAFs expressing PDPN influence proliferation of SCLC cells. Compared with control group (SCLC cells co-cultured with CAFs-Ctrl), numbers of SCLC cells co-cultured with CAFs overexpressing PDPN were decreased. Suppression of PDPN expression by shRNA in CAFs resulted in increased numbers of SCLC cells. In surgically resected human SCLC specimens, the frequency of Geminin-positive cancer cells was significantly higher in the cases with PDPN-positive CAFs than in the cases with PDPN-negative CAFs. Thus CAFs expressing PDPN inhibit growth of SCLC cells, suggesting that CAFs expressing PDPN represent a tumor inhibitory stromal cell component in SCLC.
巻・号 6(11)
ページ 9531-41
公開日 2015-4-20
DOI 10.18632/oncotarget.3371
PII 3371
PMID 25909164
PMC PMC4496236
MeSH Adenocarcinoma / pathology Aged Animals Carcinoma, Non-Small-Cell Lung / pathology Carcinoma, Small Cell / pathology* Cell Line, Tumor Coculture Techniques Female Fibroblasts / physiology* Geminin / biosynthesis Geminin / genetics Gene Expression Regulation, Neoplastic Graft Survival Heterografts Humans Lung Neoplasms / pathology* Male Membrane Glycoproteins / biosynthesis* Membrane Glycoproteins / genetics Mice Mice, SCID Middle Aged Neoplasm Proteins / biosynthesis* Neoplasm Proteins / genetics RNA Interference RNA, Small Interfering / genetics Stromal Cells / physiology* Transfection Tumor Burden Tumor Microenvironment / physiology*
IF 5.168
リソース情報
ヒト・動物細胞 PC-9(RCB4455)