RRC ID 67715
著者 Srimany A, Jayashree B, Krishnakumar S, Elchuri S, Pradeep T.
タイトル Identification of effective substrates for the direct analysis of lipids from cell lines using desorption electrospray ionization mass spectrometry.
ジャーナル Rapid Commun Mass Spectrom
Abstract RATIONALE:Various disease conditions, particularly tumours, can be understood easily by studying changes in the lipid profile of cells. While lipid profiles of tissues have been recorded by desorption electrospray ionization mass spectrometric (DESI-MS) imaging, there is paucity in standardized protocols for sample preparation involving cell cultures to generate reliable results. In this study, we report a method for the direct analysis of lipids from cultured cells by incorporating them onto Whatman 42 filter paper as a substrate for reliable DESI-MS analysis.
METHODS:The WERI-RB1 cell line was spotted on commonly used substrates for DESI-MS analysis, such as glass slides, Teflon coated glass slides, thin layer chromatography (TLC) plates, and Whatman 42 filter paper. A comparison of mass spectrometric images with two different lipids was made to understand the behaviour of different surfaces when the same sample was spotted on them. Relative intensities of different lipid peaks in the WERI-RB1 cell line were compared and relative lipid abundances were also compared across two different human retinoblastoma cell lines; WERI-RB1 and Y79.
RESULTS:The study demonstrates that good lipid signals can be obtained by DESI-MS when the cells are spotted on Whatman 42 filter paper. Tandem mass spectrometry was performed to identify the lipids as glycerophosphocholines (PC). Better lipid images from assembly of cells were obtained with distinct boundary when they were spotted on Whatman 42 filter paper than other surfaces.
CONCLUSIONS:We demonstrate the use of a simple substrate for reliable DESI-MS analysis of cultured cells. This method has the potential to understand various interactions of cells with other external agents. The current method would help in the application of DESI-MS for biology in general and medical sciences in particular.
巻・号 29(4)
ページ 349-56
公開日 2015-2-28
DOI 10.1002/rcm.7111
PMID 26406347
MeSH Cell Line, Tumor Humans Lipids / analysis* Phenethylamines / chemistry Retinoblastoma / chemistry Spectrometry, Mass, Electrospray Ionization / methods* Tandem Mass Spectrometry / methods
IF 2.2
リソース情報
ヒト・動物細胞 WERI-Rb-1(RCB2146) Y79(RCB1645)