| RRC ID |
6772
|
| Author |
Hirata H, Kawamata S, Murakami Y, Inoue K, Nagahashi A, Tosaka M, Yoshimura N, Miyamoto Y, Iwasaki H, Asahara T, Sawa Y.
|
| Title |
Coexpression of platelet-derived growth factor receptor alpha and fetal liver kinase 1 enhances cardiogenic potential in embryonic stem cell differentiation in vitro.
|
| Journal |
J Biosci Bioeng
|
| Abstract |
Nascent mesodermal cells derived from EB5 embryonic stem (ES) cells were sorted in terms of cardiogenic potential on the basis of their expression levels of platelet-derived growth factor receptor alpha (PDGFRalpha) and fetal liver kinase 1 (Flk-1). The sorted cells were cocultured with OP9 stromal cells to induce terminal differentiation into contractile cardiac colonies. A significant number of cardiac colonies were found in the Flk-1+/PDGFRalpha+ fraction. The enrichment double-positive fraction produced approximately fivefold more cardiac colonies than the Flk-1+/PDGFRalpha- fraction and 10-fold more than the Flk-1-/PDGFRalpha+ fraction. To investigate the involvement of these markers in embryonic cardiogenesis, the cells that disseminated from the E7.5-7.75 embryos were fractionated and seeded on OP9 cells. The cardiogenic potential was markedly enhanced in the Flk-1+/PDGFRalpha+ fraction. These results suggest that some of the precursor cells coexpressing these markers are selectively involved in cardiogenic events, and that the identification of ES-cell-derived precursors with these markers will contribute to the effective production of cardiomyocytes for cell therapies.
|
| Volume |
103(5)
|
| Pages |
412-9
|
| Published |
2007-5-1
|
| DOI |
10.1263/jbb.103.412
|
| PII |
S1389-1723(07)70081-6
|
| PMID |
17609155
|
| MeSH |
Animals
Biomarkers / metabolism
Cell Differentiation
Cell Line
Embryonic Development / physiology*
Embryonic Stem Cells / cytology*
Embryonic Stem Cells / physiology*
Gene Expression / physiology
Mice
Mice, Inbred ICR
Myocardial Contraction / physiology
Myocytes, Cardiac / cytology*
Myocytes, Cardiac / physiology*
Receptor, Platelet-Derived Growth Factor alpha / metabolism*
Vascular Endothelial Growth Factor Receptor-2 / metabolism*
|
| IF |
2.366
|
| Times Cited |
25
|
|
WOS Category
|
FOOD SCIENCE & TECHNOLOGY
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
|
| Resource |
| Human and Animal Cells |
OP9(RCB1124) |
