Reference - Detail
| RRC ID | 67753 |
|---|---|
| Author | Mancini SJ, Elantak L, Boned A, Espéli M, Guerlesquin F, Schiff C. |
| Title | Examination of galectin-induced lattice formation on early B-cell development. |
| Journal | Methods Mol Biol |
| Abstract |
Galectin-1 (GAL1) is a pre-B cell receptor (pre-BCR) ligand that induces pre-BCR clustering and leads to efficient pre-B cell proliferation and differentiation in the bone marrow. To study pre-BCR-GAL1 interactions and its functional consequence on the early steps of the B cell development, we combine structural nuclear magnetic resonance (NMR) approaches and B cell biology techniques. NMR is applied to identify the residues involved in pre-BCR-GAL1 interactions by monitoring chemical shift perturbations when the complex is formed. This structural information is then used at the cellular level to target specifically the complex formation during GAL1-induced pre-BCR clustering and lattice formation, using immunofluorescence techniques. Moreover, an in vivo assay was set up to study the consequence of synapse formation on the early steps of B cell development. |
| Volume | 1207 |
| Pages | 169-84 |
| Published | 2015-1-1 |
| DOI | 10.1007/978-1-4939-1396-1_11 |
| PMID | 25253140 |
| MeSH | B-Lymphocytes / cytology* Bone Marrow Cells / cytology Cell Line Fluorescent Antibody Technique / methods* Galectin 1 / metabolism* Humans Pre-B Cell Receptors / metabolism |
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | OP9(RCB1124) |