| RRC ID |
67753
|
| Author |
Mancini SJ, Elantak L, Boned A, Espéli M, Guerlesquin F, Schiff C.
|
| Title |
Examination of galectin-induced lattice formation on early B-cell development.
|
| Journal |
Methods Mol Biol
|
| Abstract |
Galectin-1 (GAL1) is a pre-B cell receptor (pre-BCR) ligand that induces pre-BCR clustering and leads to efficient pre-B cell proliferation and differentiation in the bone marrow. To study pre-BCR-GAL1 interactions and its functional consequence on the early steps of the B cell development, we combine structural nuclear magnetic resonance (NMR) approaches and B cell biology techniques. NMR is applied to identify the residues involved in pre-BCR-GAL1 interactions by monitoring chemical shift perturbations when the complex is formed. This structural information is then used at the cellular level to target specifically the complex formation during GAL1-induced pre-BCR clustering and lattice formation, using immunofluorescence techniques. Moreover, an in vivo assay was set up to study the consequence of synapse formation on the early steps of B cell development.
|
| Volume |
1207
|
| Pages |
169-84
|
| Published |
2015-1-1
|
| DOI |
10.1007/978-1-4939-1396-1_11
|
| PMID |
25253140
|
| MeSH |
B-Lymphocytes / cytology*
Bone Marrow Cells / cytology
Cell Line
Fluorescent Antibody Technique / methods*
Galectin 1 / metabolism*
Humans
Pre-B Cell Receptors / metabolism
|
| Resource |
| Human and Animal Cells |
OP9(RCB1124) |
