RRC ID 67758
Author Kawata K, Narita K, Washio A, Kitamura C, Nishihara T, Kubota S, Takeda S.
Title Odontoblast differentiation is regulated by an interplay between primary cilia and the canonical Wnt pathway.
Journal Bone
Abstract Primary cilium is a protruding cellular organelle that has various physiological functions, especially in sensory reception. While an avalanche of reports on primary cilia have been published, the function of primary cilia in dental cells remains to be investigated. In this study, we focused on the function of primary cilia in dentin-producing odontoblasts. Odontoblasts, like most other cell types, possess primary cilia, which disappear upon the knockdown of intraflagellar transport protein 88. In cilia-depleted cells, the expression of dentin sialoprotein, an odontoblastic marker, was elevated, while the deposition of minerals was slowed. This was recapitulated by the activation of canonical Wnt pathway, also decreased the ratio of ciliated cells. In dental pulp cells, as they differentiated into odontoblasts, the ratio of ciliated cells was increased, whereas the canonical Wnt signaling activity was repressed. Our results collectively underscore the roles of primary cilia in regulating odontoblastic differentiation through canonical Wnt signaling. This study implies the existence of a feedback loop between primary cilia and the canonical Wnt pathway.
Volume 150
Pages 116001
Published 2021-9-1
DOI 10.1016/j.bone.2021.116001
PII S8756-3282(21)00163-0
PMID 33975031
MeSH Cell Differentiation Cilia Dental Pulp Odontoblasts* Wnt Signaling Pathway*
IF 4.147
DNA material CSII-CMV-MCS-IRES2-Bsd (RDB04385) pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394)