RRC ID 6779
Author Yokoe T, Tanaka F, Mimori K, Inoue H, Ohmachi T, Kusunoki M, Mori M.
Title Efficient identification of a novel cancer/testis antigen for immunotherapy using three-step microarray analysis.
Journal Cancer Res
Abstract Advanced technology in molecular biology has provided us powerful tools for the diagnosis and treatment for cancer. We herein adopted a new methodology to identify a novel cancer/testis (CT) antigen with high frequency of expression in colorectal cancer as follows: (a) combining laser microdissection and cDNA microarray was used to analyze the gene expression profile of colorectal cancer cells; (b) genes overexpressed in testis and underexpressed in normal colon epithelium were analyzed using cDNA microarray; and (c) the gene expression profile of colorectal cancer cells was compared with that of normal testis. Using this methodology, we selected 38 candidates for CT antigen. Among these genes, we identified a novel CT antigen, serine/threonine kinase 31 (STK31), which was previously reported as a gene expressed in spermatogonia. Reverse transcription-PCR analysis showed that STK31 gene expression levels in cancer samples were significantly higher (P < 0.0001) than those in normal samples. The STK31 gene was frequently expressed not only in colorectal cancer but also in gastric and esophageal cancer. Moreover, STK31 peptide was able to elicit specific CTLs and induced CTLs lysed either peptide-loading or endogenously STK31-expressing target cells. These results showed that the new methodology in this study facilitated identification of CT antigens and that STK31 may be a candidate for cancer immunotherapy against gastrointestinal cancer.
Volume 68(4)
Pages 1074-82
Published 2008-2-15
DOI 10.1158/0008-5472.CAN-07-0964
PII 68/4/1074
PMID 18281482
MeSH Antigens, Neoplasm / biosynthesis Antigens, Neoplasm / genetics Antigens, Neoplasm / immunology* Colorectal Neoplasms / enzymology Colorectal Neoplasms / genetics Colorectal Neoplasms / immunology* Gene Expression HLA-A Antigens / genetics HLA-A Antigens / immunology HLA-A2 Antigen Humans Immunotherapy / methods Male Melanoma-Specific Antigens Neoplasm Proteins Oligonucleotide Array Sequence Analysis Oligopeptides / immunology Protein Serine-Threonine Kinases / biosynthesis Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / immunology* RNA, Messenger / biosynthesis RNA, Messenger / genetics T-Lymphocytes / immunology Testis / enzymology Testis / immunology
IF 9.727
Times Cited 29
Human and Animal Cells T2(RCB1932)