Reference - Detail
| RRC ID | 6785 |
|---|---|
| Author | Wakao H, Wakao R, Sakata S, Iwabuchi K, Oda A, Fujita H. |
| Title | In vitro induction of natural killer T cells from embryonic stem cells prepared using somatic cell nuclear transfer. |
| Journal | FASEB J |
| Abstract |
The ectopic expression of the Notch receptor ligand delta-like 1 on stromal cells allows the induction of T cells from embryonic stem cells (ESCs). However, these in vitro-generated T cells are not transplantable because they are too immature to mount an immune response in an immunocompromised animal. We efficiently generated a subset of T cells called invariant natural killer T (iNKT) cells from ESCs derived from peripheral iNKT cells using somatic cell nuclear transfer (ntESCs). These iNKT cells matured autonomously in vivo and exhibited an adjuvant effect accompanying the production of interferon-gamma in an antigen-specific manner. This adjuvant effect culminated in the inhibition of inoculated tumor cell growth. Our results indicate that ntESC-derived iNKT cells are transplantable lymphocytes that will be beneficial for the induction of immune tolerance and the treatment of autoimmune diseases, tumors, and infections. |
| Volume | 22(7) |
| Pages | 2223-31 |
| Published | 2008-7-1 |
| DOI | 10.1096/fj.07-104687 |
| PII | fj.07-104687 |
| PMID | 18323403 |
| MeSH | Animals CD3 Complex / genetics Cell Nucleus / immunology* DNA-Binding Proteins / genetics Embryonic Stem Cells / cytology Embryonic Stem Cells / immunology* GATA3 Transcription Factor / genetics Humans Killer Cells, Natural / immunology* Nuclear Proteins / genetics Nuclear Transfer Techniques Receptors, Interleukin-7 / genetics Reverse Transcriptase Polymerase Chain Reaction |
| IF | 4.966 |
| Times Cited | 6 |
| WOS Category | BIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 7 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | OP9(RCB1124) |