| RRC ID |
6786
|
| 著者 |
Alitheen NB, Manaf AA, Yeap SK, Shuhaimi M, Nordin L, Mashitoh AR.
|
| タイトル |
Immunomodulatory effects of damnacanthal isolated from roots of Morinda elliptica.
|
| ジャーナル |
Pharm Biol
|
| Abstract |
Morinda elliptica Ridley (Rubiaceae) has been used traditionally as a medicine to treat various diseases in Malaysia and southeast Asia. In the present study we investigated the immunomodulatory effects of damnacanthal isolated from the roots of Morinda elliptica. The immunomodulatory effect of this compound was evaluated by using the lymphocyte proliferation assay with mouse thymocytes and human peripheral blood mononuclear cells (PBMC). In addition, the effect of the compound on PBMC cell cycle progression was studied by using flow cytometry. The production of human interleukin-2 and human inteleukin-12 cytokines was also assessed using the enzyme linked immunosorbent assay (ELISA) technique. The lymphocyte proliferation assay showed that damnacanthal was able to activate mouse thymocytes and PBMC at a low concentration (0.468 microg/mL). Moreover, the production of human interleukin-2 and human interleukin-12 cytokines in the culture supernatant from damnacanthal activated lymphocytes was markedly up-regulated at 24 h and sustained until 72 h with a slight decrease with time. A positive correlation was found between the level of these two cytokines and the MTT-based proliferation assay. Based on the above results, damnacanthal can act as an immunomodulatory agent which may be very useful for maintaining a healthy immune system.
|
| 巻・号 |
48(4)
|
| ページ |
446-52
|
| 公開日 |
2010-4-1
|
| DOI |
10.3109/13880200903168031
|
| PMID |
20645725
|
| MeSH |
Adjuvants, Immunologic / isolation & purification
Adjuvants, Immunologic / pharmacology*
Animals
Anthraquinones / isolation & purification
Anthraquinones / pharmacology*
Cell Proliferation / drug effects
Culture Media
Dose-Response Relationship, Drug
Humans
Interleukin-12 / biosynthesis
Interleukin-12 / immunology
Interleukin-2 / biosynthesis
Interleukin-2 / immunology
Leukocytes, Mononuclear / drug effects
Leukocytes, Mononuclear / immunology
Lymphocyte Activation / drug effects
Lymphocyte Activation / immunology
Lymphocytes / drug effects
Lymphocytes / immunology
Malaysia
Male
Mice
Mice, Inbred ICR
Morinda / chemistry*
Plant Roots / chemistry
Thymus Gland / cytology
Thymus Gland / drug effects
Thymus Gland / immunology
Time Factors
|
| 引用数 |
2
|
|
WOS 分野
|
HEMATOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
HL-60 |
