論文 - 詳細
| RRC ID | 6787 |
|---|---|
| 著者 | Matsumoto Y, Iwakawa M, Furusawa Y, Ishikawa K, Aoki M, Imadome K, Matsumoto I, Tsujii H, Ando K, Imai T. |
| タイトル | Gene expression analysis in human malignant melanoma cell lines exposed to carbon beams. |
| ジャーナル | Int J Radiat Biol |
| Abstract |
PURPOSE:To elucidate the molecular changes in response to carbon beams (C-ions) in melanoma. MATERIALS AND METHODS:We examined expression profiles of 6 melanoma cell lines exposed to C-ions or X-rays with 2 Gy using single-color microarrays. RESULTS:Twenty-two genes, including nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA), responded to C-ions in all six cell lines, based on analysis of variance (ANOVA) filtering (p < 0.001). We found 173 genes that responded in common to C-ions in four cell lines. We identified many down-regulated genes including the cell cycle - related genes that were more responsive to C-ions than X-rays. In contrast, most of the up-regulated genes including the tumor protein p53 (p53) target genes responded to both C-ions and X-rays. C-ions induced G2/M arrest significantly more than X-rays at 30 h (p < 0.05). CONCLUSION:Our findings suggest that down-regulation of gene expression plays a key role in the response to C-ions. Regulation of cell cycle - related genes and induction of prolonged G2/M arrest may be responsible for the extra sensitivity to C-ions, whereas p53-related genes may have similar roles in the sensitivities to both C-ions and X-rays. |
| 巻・号 | 84(4) |
| ページ | 299-314 |
| 公開日 | 2008-4-1 |
| DOI | 10.1080/09553000801953334 |
| PII | 791889986 |
| PMID | 18386195 |
| MeSH | Carbon Isotopes* Cell Line, Tumor Dose-Response Relationship, Radiation Gene Expression Regulation, Neoplastic / radiation effects* Heavy Ions* Humans Melanoma / metabolism* Neoplasm Proteins / metabolism* Radiation Dosage |
| IF | 2.368 |
| 引用数 | 32 |
| WOS 分野 | NUCLEAR SCIENCE & TECHNOLOGY BIOLOGY RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING |
| リソース情報 | |
| ヒト・動物細胞 | COLO 679(RCB0989) HMV-II(RCB0777) |