Reference - Detail
| RRC ID | 67871 |
|---|---|
| Author | Tanahashi H, Tian QB, Hara Y, Sakagami H, Endo S, Suzuki T. |
| Title | Polyhydramnios in Lrp4 knockout mice with bilateral kidney agenesis: Defects in the pathways of amniotic fluid clearance. |
| Journal | Sci Rep |
| Abstract |
Amniotic fluid volume during mid-to-late gestation depends mainly on the urine excretion from the foetal kidneys and partly on the fluid secretion from the foetal lungs during foetal breathing-like movements. Urine is necessary for foetal breathing-like movements, which is critical for foetal lung development. Bilateral renal agenesis and/or obstruction of the urinary tract lead to oligohydramnios, which causes infant death within a short period after birth due to pulmonary hypoplasia. Lrp4, which functions as an agrin receptor, is essential for the formation of neuromuscular junctions. Herein, we report novel phenotypes of Lrp4 knockout (Lrp4(-/-)) mice. Most Lrp4(-/-) foetuses showed unilateral or bilateral kidney agenesis, and Lrp4 knockout resulted in polyhydramnios. The loss of Lrp4 compromised foetal swallowing and breathing-like movements and downregulated the expression of aquaporin-9 in the foetal membrane and aquaporin-1 in the placenta, which possibly affected the amniotic fluid clearance. These results suggest that amniotic fluid removal was compromised in Lrp4(-/-) foetuses, resulting in polyhydramnios despite the impairment of urine production. Our findings indicate that amniotic fluid removal plays an essential role in regulating the amniotic fluid volume. |
| Volume | 6 |
| Pages | 20241 |
| Published | 2016-2-5 |
| DOI | 10.1038/srep20241 |
| PII | srep20241 |
| PMID | 26847765 |
| PMC | PMC4742865 |
| MeSH | Amnion / metabolism Amniotic Fluid / chemistry Amniotic Fluid / metabolism* Animals Aquaporin 1 / genetics Aquaporin 1 / metabolism Aquaporins / genetics Aquaporins / metabolism Electrolytes / chemistry Female Kidney / metabolism* Kidney / pathology LDL-Receptor Related Proteins Lung / metabolism Male Mice Mice, Inbred C57BL Mice, Knockout Myometrium / metabolism Myometrium / pathology Osmolar Concentration Placenta / metabolism Polyhydramnios / etiology* Polyhydramnios / metabolism Pregnancy Pulmonary Surfactant-Associated Protein A / metabolism Receptors, LDL / deficiency Receptors, LDL / genetics* |
| IF | 3.998 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | C2C12(RCB0987) Hep G2 |