論文 - 詳細
| RRC ID | 6788 |
|---|---|
| 著者 | Mori R, Sakai Y, Nakazawa K. |
| タイトル | Micropatterned organoid culture of rat hepatocytes and HepG2 cells. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
The culture of liver cell organoids (multicellular aggregates) such as spheroids or cylindroids, which can strongly express liver functions, has been advocated as a useful technique that has advantages over monolayer culture. This paper describes a micropatterning technique for obtaining spheroids and cylindroids by using rat hepatocytes or HepG2 cells. We developed culture chips that comprised multiple, circular or rectangular microwells; the bottom surface of each microwell was modified with collagen to create a cell adhesion area, and the entire microwell, excluding the collagen-coated spots, was modified with polyethylene glycol (PEG) to create a nonadhesive area. Rat hepatocytes and HepG2 cells formed uniform spheroids and cylindroids on the circular and rectangular chips, respectively. Consequently, two-dimensional micropatterned chips containing homogeneous spheroids or cylindroids were generated. The expression of liver functions (protein secretion and ammonia removal) was greater in the spheroids and cylindroids than in the monolayer culture, and this expression was maintained for at least 2 weeks of culture. Thus, this chip technology has potential for use in various applications that involve organoid culture. |
| 巻・号 | 106(3) |
| ページ | 237-42 |
| 公開日 | 2008-9-1 |
| DOI | 10.1263/jbb.106.237 |
| PII | S1389-1723(08)70174-9 |
| PMID | 18929998 |
| MeSH | Animals Cell Culture Techniques / methods* Cell Line Cell Proliferation Hepatocytes / cytology* Hepatocytes / physiology* Liver / cytology* Liver / growth & development* Liver, Artificial* Organ Culture Techniques / methods* Rats Tissue Engineering / methods* |
| IF | 2.366 |
| 引用数 | 35 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 26 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB1648) |