RRC ID 67886
Author Guo J, Yang D, Okamura H, Teramachi J, Ochiai K, Qiu L, Haneji T.
Title Calcium hydroxide suppresses Porphyromonas endodontalis lipopolysaccharide-induced bone destruction.
Journal J Dent Res
Abstract Porphyromonas endodontalis and its main virulence factor, lipopolysaccharide (LPS), are associated with the development of periapical diseases and alveolar bone loss. Calcium hydroxide is commonly used for endodontic therapy. However, the effects of calcium hydroxide on the virulence of P. endodontalis LPS and the mechanism of P. endodontalis LPS-induced bone destruction are not clear. Calcium hydroxide rescued the P. endodontalis LPS-suppressed viability of MC3T3-E1 cells and activity of nuclear factor-κB (NF-κB) in these cells, resulting in the reduced expression of interleukin-6 and tumor necrosis factor-α. In addition, calcium hydroxide inhibited P. endodontalis LPS-induced osteoclastogenesis by decreasing the activities of NF-κB, p38, and ERK1/2 and the expression of nuclear factor of activated T-cell cytoplasmic 1 in RAW264.7 cells. Calcium hydroxide also rescued the P. endodontalis LPS-induced osteoclastogenesis and bone destruction in mouse calvaria. Taken together, our present results indicate that calcium hydroxide suppressed bone destruction by attenuating the virulence of P. endodontalis LPS on bone cells.
Volume 93(5)
Pages 508-13
Published 2014-5-1
DOI 10.1177/0022034514526886
PII 0022034514526886
PMID 24603641
MeSH 3T3 Cells Acid Phosphatase / analysis Animals Biomarkers / analysis Bone Resorption / prevention & control Calcium Hydroxide / pharmacology* Cell Line Cell Survival / drug effects Extracellular Signal-Regulated MAP Kinases / drug effects Interleukin-6 / analysis Isoenzymes / analysis Lipopolysaccharides / antagonists & inhibitors Lipopolysaccharides / pharmacology* Mice Mitogen-Activated Protein Kinase 3 / drug effects NF-kappa B / drug effects NFATC Transcription Factors / drug effects Osteoblasts / drug effects* Osteoclasts / drug effects* Porphyromonas endodontalis / drug effects* Porphyromonas endodontalis / pathogenicity Root Canal Irrigants / pharmacology* Skull / drug effects Tartrate-Resistant Acid Phosphatase Tumor Necrosis Factor-alpha / drug effects X-Ray Microtomography p38 Mitogen-Activated Protein Kinases / drug effects
IF 4.914
Human and Animal Cells MC3T3-E1(RCB1126)