論文 - 詳細
| RRC ID | 67901 |
|---|---|
| 著者 | Fujii M, Kawai K, Egami Y, Araki N. |
| タイトル | Dissecting the roles of Rac1 activation and deactivation in macropinocytosis using microscopic photo-manipulation. |
| ジャーナル | Sci Rep |
| Abstract |
Macropinocytosis, a fluid-phase endocytosis, is a crucial pathway for antigen uptake and presentation in macrophages. We attempted to characterise the activation and deactivation of a small GTPase molecular switch, Rac1, in macropinocytosis using microscopic photo-manipulation. Expression of genetically encoded photoactivatable-Rac1 (PA-Rac1) in RAW264 macrophages enabled the local, reversible control of macropinocytosis using blue laser irradiation. Marked membrane ruffling and unclosed pre-macropinosomes were observed in the irradiated region of macrophages under the persistent activation of PA-Rac1. Although phosphatidylinositol 4,5-bisphosphate and actin were also localised to this region, the recruitment of maturating endosome markers, such as phosphatidylinositol 3-phosphate and Rab21, was restricted until PA-Rac1 deactivation. After deactivating PA-Rac1 by ceasing irradiation, membrane ruffling immediately receded, and the macropinosomes acquired maturation markers. These data suggest that activation of Rac1 is sufficient to induce membrane ruffling and macropinocytic cup formation, but subsequent deactivation of Rac1 is required for macropinosome closure and further maturation. |
| 巻・号 | 3 |
| ページ | 2385 |
| 公開日 | 2013-1-1 |
| DOI | 10.1038/srep02385 |
| PII | srep02385 |
| PMID | 23924974 |
| PMC | PMC3737501 |
| MeSH | Animals Cell Line Macrophage Activation / physiology* Macrophages / physiology* Mice Optical Tweezers* Pinocytosis / physiology* rac1 GTP-Binding Protein / chemistry* rac1 GTP-Binding Protein / radiation effects* |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |