論文 - 詳細
| RRC ID | 67916 |
|---|---|
| 著者 | Kovacs-Nolan J, Kanatani H, Nakamura A, Ibuki M, Mine Y. |
| タイトル | β-1,4-mannobiose stimulates innate immune responses and induces TLR4-dependent activation of mouse macrophages but reduces severity of inflammation during endotoxemia in mice. |
| ジャーナル | J Nutr |
| Abstract |
β-1,4-Mannobiose (MNB) has been shown to exert prebiotic activity and modulate mucosal gene expression. In this study, the immune-modulating effect of MNB in healthy and endotoxemic mice and its role in Toll-like receptor (TLR) 2/4-mediated macrophage activation were investigated. Mice were supplemented daily with MNB (0, 5, 10, or 25 mg/kg) for 14 d. To examine the effect of MNB during endotoxemia, mice were supplemented with or without MNB (25 mg/kg) for 14 d, followed by challenge with intraperitoneal LPS or saline. MNB induced expression of both T helper (Th) 1- and Th2-type cytokines in the ileum (P < 0.05) and increased fecal IgA production and splenic NK cell activity (P < 0.05) in healthy mice. In endotoxemic mice, MNB reduced the expression of Tnfa, Il-6, iNos (P < 0.05), and Il-10 (P < 0.05), and reduced LPS-induced weight loss but increased Ifng, Il-12p40, Il-5, and Ifna expression (P < 0.05) and NK cell activity relative to positive control (LPS) mice. Treatment of RAW 264.7 macrophages with MNB induced TNF-α and IL-6 secretion (P < 0.05), and this effect was abrogated by inhibiting TLR4, but not TLR2, signaling. Pretreatment of RAW 264.7 cells with MNB induced tolerance to TLR2 and TLR4 agonists, reducing TNF-α production (P < 0.05) upon secondary stimulation with LPS or lipoteichoic acid. These results indicate that MNB can modulate intestinal and systemic immune responses in healthy and endotoxemic mice and prevent LPS-induced immune suppression, as well as directly stimulating innate immune mechanisms in vitro as a TLR4 agonist. |
| 巻・号 | 143(3) |
| ページ | 384-91 |
| 公開日 | 2013-3-1 |
| DOI | 10.3945/jn.112.167866 |
| PII | jn.112.167866 |
| PMID | 23343679 |
| MeSH | Adjuvants, Immunologic / pharmacology Adjuvants, Immunologic / therapeutic use* Animals Cytokines / metabolism Dietary Supplements Endotoxemia / drug therapy* Endotoxemia / immunology Endotoxemia / metabolism Feces / chemistry Female Ileum / drug effects Ileum / metabolism Immunity, Innate / drug effects* Immunoglobulin A / metabolism Inflammation / drug therapy* Inflammation / immunology Inflammation / metabolism Inflammation Mediators / metabolism* Lipopolysaccharides Macrophages / drug effects* Mannans / pharmacology Mannans / therapeutic use* Mice Mice, Inbred BALB C Prebiotics Severity of Illness Index Sodium Chloride Spleen / drug effects Spleen / immunology Teichoic Acids Th1 Cells / drug effects Th2 Cells / drug effects Toll-Like Receptor 4 / metabolism |
| IF | 4.281 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | YAC-1(RCB1165) |