Reference - Detail
| RRC ID | 6793 |
|---|---|
| Author | Chen J, Uto T, Tanigawa S, Kumamoto T, Fujii M, Hou DX. |
| Title | Expression profiling of genes targeted by bilberry (Vaccinium myrtillus) in macrophages through DNA microarray. |
| Journal | Nutr Cancer |
| Abstract |
To evaluate the anti-inflammatory function and underlying genes targeted by bilberry, gene expression profiling through DNA microarray was performed on bilberry extract-treated macrophages. Among 22,050 oligonucleotides, the expression levels of 998 genes were increased by > or = twofold in lipopolysaccharide (LPS)-activated RAW264 cells, 358 gene signals of which were attenuated by bilberry extract (> or = 1.5-fold). Expression levels of 2,086 genes were decreased by > or = twofold in LPS-activated cells, of which 939 gene signals were enhanced by bilberry extract (> or = 1.5-fold). Utilizing Panther group analysis, 308 genes affected by bilberry extract were classified into 43 categories relating to biological processes (97), molecular functions (186), and signaling pathways (26) with > or = 1.5-fold change. The genes categorized as "defense, inflammatory response, cytokines activities, and receptor activities" were further identified, and some of them were confirmed by real-time polymerase chain reaction. The DNA microarray results provide a molecular basis for the anti-inflammatory effects of bilberry. |
| Volume | 60 Suppl 1 |
| Pages | 43-50 |
| Published | 2008-1-1 |
| DOI | 10.1080/01635580802381279 |
| PII | 905309992 |
| PMID | 19003580 |
| MeSH | Animals Anthocyanins / pharmacology* Anti-Inflammatory Agents / pharmacology* Cell Line Gene Expression Profiling* Lipopolysaccharides / pharmacology Macrophages / drug effects* Macrophages / metabolism Mice Microarray Analysis / methods* Plant Extracts Vaccinium myrtillus |
| IF | 2.363 |
| Times Cited | 18 |
| WOS Category | NUTRITION & DIETETICS ONCOLOGY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |