| RRC ID |
6794
|
| 著者 |
Tsuruoka C, Suzuki M, Hande MP, Furusawa Y, Anzai K, Okayasu R.
|
| タイトル |
The difference in LET and ion species dependence for induction of initially measured and non-rejoined chromatin breaks in normal human fibroblasts.
|
| ジャーナル |
Radiat Res
|
| Abstract |
We studied the LET and ion species dependence of the induction of chromatin breaks measured immediately after irradiation as initially measured breaks and after 24 h postirradiation incubation (37 degrees C) as non-rejoined breaks in normal human fibroblasts with different heavy ions, such as carbon, neon, silicon and iron, generated by the Heavy Ion Medical Accelerator in Chiba (HIMAC) at the National Institute of Radiological Science (NIRS). Chromatin breaks were measured as an excess number of fragments of prematurely condensed chromosomes using premature chromosome condensation (PCC). The results showed that the number of excess fragments per cell per Gy for initially measured chromatin breaks was dependent on LET in the range from 13.3 to 113.1 keV/mum but was not dependent on ion species. On the other hand, the number of non-rejoined chromatin breaks detected after 24 h postirradiation incubation was clearly dependent on both LET and ion species. No significant difference was observed in the cross section for initially measured breaks, but a statistically significant difference was observed in the cross section for non-rejoined breaks among carbon, neon, silicon and iron ions. This suggests that the LET-dependent structure in the biological effects is reflected in biological consequences of repair processes.
|
| 巻・号 |
170(2)
|
| ページ |
163-71
|
| 公開日 |
2008-8-1
|
| DOI |
10.1667/RR1279.1
|
| PII |
RR1279
|
| PMID |
18666815
|
| MeSH |
Cell Line
Chromatin / genetics*
Chromatin / radiation effects*
DNA Breaks*
DNA Repair / genetics*
DNA Repair / radiation effects*
Dose-Response Relationship, Radiation
Fibroblasts / physiology
Fibroblasts / radiation effects*
Heavy Ions
Humans
Linear Energy Transfer / physiology*
Linear Energy Transfer / radiation effects
Radiation Dosage
|
| IF |
2.657
|
| 引用数 |
24
|
|
WOS 分野
|
BIOLOGY
RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING
BIOPHYSICS
|
| リソース情報 |
| ヒト・動物細胞 |
|
