Reference - Detail
| RRC ID | 67941 |
|---|---|
| Author | Miki T, Iguchi M, Akiba K, Hosono M, Sobue T, Danbara H, Okada N. |
| Title | Chromobacterium pathogenicity island 1 type III secretion system is a major virulence determinant for Chromobacterium violaceum-induced cell death in hepatocytes. |
| Journal | Mol Microbiol |
| Abstract |
Chromobacterium violaceum is a Gram-negative bacterium that causes fatal septicaemia in humans and animals. C. violaceum ATCC 12472 possesses genes associated with two distinct type III secretion systems (T3SSs). One of these systems is encoded by Chromobacterium pathogenicity islands 1 and 1a (Cpi-1/-1a), another is encoded by Chromobacterium pathogenicity island 2 (Cpi-2). Here we show that C. violaceum causes fulminant hepatitis in a mouse infection model, and Cpi-1/-1a-encoded T3SS is required for its virulence. In addition, using C. violaceum strains with defined mutations in the genes that encode the Cpi-1/-1a or Cpi-2 locus in combination with cultured mammalian cell lines, we found that C. violaceum is able to induce cytotoxicity in a Cpi-1/-1a-dependent manner. Characterization of Chromobacterium-induced cytotoxicity revealed that cell lysis by C. violaceum infection involves the formation of pore structures on the host cell membrane, as demonstrated by protection by cytotoxicity in the presence of osmoprotectants. Finally, we demonstrated that CipB, a Cpi-1/-1a effector, is implicated in translocator-mediated pore formation and the ability of CipB to form a pore is essential for Chromobacterium-induced cytotoxicity. These results strongly suggest that Cpi-1/-1a-encoded T3SS is a virulence determinant that causes fatal infection by the induction of cell death in hepatocytes. |
| Volume | 77(4) |
| Pages | 855-72 |
| Published | 2010-8-1 |
| DOI | 10.1111/j.1365-2958.2010.07248.x |
| PII | MMI7248 |
| PMID | 20545857 |
| MeSH | Animals Bacterial Secretion Systems* Bodily Secretions Cell Death Chromobacterium / genetics* Chromobacterium / growth & development* Chromobacterium / pathogenicity Disease Models, Animal Genomic Islands* Gram-Negative Bacterial Infections / microbiology Gram-Negative Bacterial Infections / pathology Hepatitis, Animal / microbiology Hepatitis, Animal / pathology Hepatocytes / microbiology Hepatocytes / physiology Mice Virulence Virulence Factors / genetics Virulence Factors / metabolism* |
| IF | 3.418 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | F1000Research |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | Hepa 1-6(RCB1638) Hep G2(RCB1648) |